Ade4 yeast mutant strain酵母突变菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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Ade4 yeast mutant strain酵母突变菌株
BioVector-YM4915 Ade4 yeast mutant strain ADE4 encodes 5-phosphoribosyl-1-pyrophosphate amidotransferase (EC 2.4.2.14), also known as amidophosphoribosyltransferase or PRPPAT (phosphoribosylpyrophosphate amidotransferase), which catalyzes the first step of the de novo purine nucleotide biosynthetic pathway (4, 5). Expression of ADE4, and of other genes in the yeast ADE regulon, is repressed by the presence of purine bases and derepressed in the absence of purines through the action of transcription factors such as Bas1p, Pho2p, and Gcn4p (6, 7). Bas1p binds several ADE promoters (ADE1, ADE2, ADE4, and ADE5,7) and DNA-bound Bas1p recruits Pho2p to activate ADE genes in S. cerevisiae (6). Gcn4p also regulates the expression of these genes (8). There are three Gcn4p responsive elements (GCREs) in the 5'-flanking region of ADE4 (9). Two GCREs are essential to synergistically activate ADE4 transcription by binding Gcn4p (9). The distal GCRE1 is also required for basal transcription of ADE4 (9). Ade4p forms cytoplasmic foci in the absence of adenine, and cycling between punctate and diffuse cellular distribution can be controlled by adenine subtraction and addition (10).ade4 null mutants exhibit adenine auxotrophy, decreased resistance to ethanol, and slow growth (11, 12, 13). Cisplatin resistance is conferred either by an ADE4 activation mutation, which causes constitutive AMP synthesis and hypoxanthine excretion, or by overexpression of ADE4, which increases de novo synthesis of purine nucleotides (14). Mutations in ADE4 are epistatic with mutations in ADE2, which encodes encodes aminoimidazole ribonucleotide-carboxylase (AIR-carboxylase), an enzyme catalyzing the sixth step of purine nucleotide biosynthesis (15), and with mutations in ADE13, which encodes adenylosuccinate lyase, an enzyme catalyzing steps 8 and 12 of the de novo purine pathway (16).Orthologs of Ade4p have been identified in Bacillus subtilis, Escherichia coli, Ashbya gossypii, Schizosaccharomyces pombe, Gallus gallus, Rattus rattus, and Homo sapiens (1, 7, 17, 18, 19). The human PRPPAT cDNA complements the S. cerevisiae ade4 null mutant (20).Standard NameADE4 Strain IDBioVector-YM4915Feature TypeORF , VerifiedDescriptionPhosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferaseName DescriptionADEnine requiringADE4 Location: Chromosome XIII 865559..867091Subfeatures - S288CFeature Relative Coordinates Coordinates Coord. Version Seq. VersionCDS 1..1533 chrXIII:867091..865559 2011-02-03 1996-07-31Protein Length (a.a.) 510Mol. Weight (Da) 56713.0Isoelectric Point 6.2Median Abundance (molecules/cell) 11336 +/- 5602Alleles: ade4-ΔGene OntologyAmidophosphoribosyltransferase involved in 'de novo' IMP (inosine monophosphate) biosynthetic process; localized to the cytoplasmMolecular Function·enables amidophosphoribosyltransferase activity (IDA, IMP)Biological Process·involved in 'de novo' IMP biosynthetic process (IMP)Cellular Component·part of cytoplasm (HDA)Pathways·superpathway of purine nucleotides de novo biosynthesis II·superpathway of purine biosynthesis and salvage pathways·superpathway of histidine, purine, and pyrimidine biosynthesis·de novo biosynthesis of purine nucleotidesPhenotypeNon-essential gene; null mutant displays adenine auxotrophy and resistance to cisplatin; in large-scale studies null mutant is haploinsufficient and shows shortened chronological lifespan
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
BioVector-YM4915 Ade4 yeast mutant strain ADE4 encodes 5-phosphoribosyl-1-pyrophosphate amidotransferase (EC 2.4.2.14), also known as amidophosphoribosyltransferase or PRPPAT (phosphoribosylpyrophosphate amidotransferase), which catalyzes the first step of the de novo purine nucleotide biosynthetic pathway (4, 5). Expression of ADE4, and of other genes in the yeast ADE regulon, is repressed by the presence of purine bases and derepressed in the absence of purines through the action of transcription factors such as Bas1p, Pho2p, and Gcn4p (6, 7). Bas1p binds several ADE promoters (ADE1, ADE2, ADE4, and ADE5,7) and DNA-bound Bas1p recruits Pho2p to activate ADE genes in S. cerevisiae (6). Gcn4p also regulates the expression of these genes (8). There are three Gcn4p responsive elements (GCREs) in the 5'-flanking region of ADE4 (9). Two GCREs are essential to synergistically activate ADE4 transcription by binding Gcn4p (9). The distal GCRE1 is also required for basal transcription of ADE4 (9). Ade4p forms cytoplasmic foci in the absence of adenine, and cycling between punctate and diffuse cellular distribution can be controlled by adenine subtraction and addition (10).ade4 null mutants exhibit adenine auxotrophy, decreased resistance to ethanol, and slow growth (11, 12, 13). Cisplatin resistance is conferred either by an ADE4 activation mutation, which causes constitutive AMP synthesis and hypoxanthine excretion, or by overexpression of ADE4, which increases de novo synthesis of purine nucleotides (14). Mutations in ADE4 are epistatic with mutations in ADE2, which encodes encodes aminoimidazole ribonucleotide-carboxylase (AIR-carboxylase), an enzyme catalyzing the sixth step of purine nucleotide biosynthesis (15), and with mutations in ADE13, which encodes adenylosuccinate lyase, an enzyme catalyzing steps 8 and 12 of the de novo purine pathway (16).Orthologs of Ade4p have been identified in Bacillus subtilis, Escherichia coli, Ashbya gossypii, Schizosaccharomyces pombe, Gallus gallus, Rattus rattus, and Homo sapiens (1, 7, 17, 18, 19). The human PRPPAT cDNA complements the S. cerevisiae ade4 null mutant (20).Standard NameADE4 Strain IDBioVector-YM4915Feature TypeORF , VerifiedDescriptionPhosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferaseName DescriptionADEnine requiringADE4 Location: Chromosome XIII 865559..867091Subfeatures - S288CFeature Relative Coordinates Coordinates Coord. Version Seq. VersionCDS 1..1533 chrXIII:867091..865559 2011-02-03 1996-07-31Protein Length (a.a.) 510Mol. Weight (Da) 56713.0Isoelectric Point 6.2Median Abundance (molecules/cell) 11336 +/- 5602Alleles: ade4-ΔGene OntologyAmidophosphoribosyltransferase involved in 'de novo' IMP (inosine monophosphate) biosynthetic process; localized to the cytoplasmMolecular Function·enables amidophosphoribosyltransferase activity (IDA, IMP)Biological Process·involved in 'de novo' IMP biosynthetic process (IMP)Cellular Component·part of cytoplasm (HDA)Pathways·superpathway of purine nucleotides de novo biosynthesis II·superpathway of purine biosynthesis and salvage pathways·superpathway of histidine, purine, and pyrimidine biosynthesis·de novo biosynthesis of purine nucleotidesPhenotypeNon-essential gene; null mutant displays adenine auxotrophy and resistance to cisplatin; in large-scale studies null mutant is haploinsufficient and shows shortened chronological lifespan
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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