Alistipes putredinis strain另枝菌属菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:Alistipes putredinis
- 产 地:北京
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- 联系人:Dr.Xu, Biovector NTCC Inc.
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Alistipes putredinis strain另枝菌属菌株
This is a type strain and a genome sequenced strain that has been sequenced as a reference genome for the NIH Human Microbiome Project.Product categoryBacteriaProduct typeAnaerobeStrain designationVPI 3293 [1Ando, CCUG 45780, CIP 104286, DSM 17216]Type strainYesGenome sequenced strainYesIsolation sourceFecesProduct formatFreeze-driedBSL:2Medium 260: Trypticase soy agar/broth with defibrinated sheep bloodTemperature37°CAtmosphereAnaerobicHandling procedureOpen vial according to enclosed instructions or visit www.atcc.org for instructions.Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of Anaerobe Systems Chopped Meat broth. Aseptically transfer the entire contents to a 5-6 mL tube of Anaerobe Systems Chopped Meat broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.Incubate in an anaerobic atmosphere at 37°C for 3-5 days. Incubate one agar plate aerobically at 37°C to check for contamination. ANAEROBIC CONDITIONS:Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in an anaerobic chamberLoose screw caps on test tubes in an activated anaerobic gas pack jarUse of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained Handling notesAnaerobe Systems Chopped Meat broth is recommended for growth. Brucella blood agar may be used in place of TSA+5% SB agar.Additional information on this culture is availableQuality control specificationsVerification methodWhole-genome SequencingHistoryDeposited asBacteroides putredinis (Weinberg et al.) KellyDepositorsEP CatoChain of custodyBioVector<-- EP Cato <-- S Suzuki 1 AndoType of isolateHumanSpecial collectionHuman Microbiome Project (HMP)Cross referencesGenBank L16497 Bacteroides putredinis ATCC 29800 16S ribosomal RNA gene, complete sequence.GenBank ABFK02000000 Alistipes putredinis DSM 17216, whole genome shotgun sequencing project
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
This is a type strain and a genome sequenced strain that has been sequenced as a reference genome for the NIH Human Microbiome Project.Product categoryBacteriaProduct typeAnaerobeStrain designationVPI 3293 [1Ando, CCUG 45780, CIP 104286, DSM 17216]Type strainYesGenome sequenced strainYesIsolation sourceFecesProduct formatFreeze-driedBSL:2Medium 260: Trypticase soy agar/broth with defibrinated sheep bloodTemperature37°CAtmosphereAnaerobicHandling procedureOpen vial according to enclosed instructions or visit www.atcc.org for instructions.Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of Anaerobe Systems Chopped Meat broth. Aseptically transfer the entire contents to a 5-6 mL tube of Anaerobe Systems Chopped Meat broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.Incubate in an anaerobic atmosphere at 37°C for 3-5 days. Incubate one agar plate aerobically at 37°C to check for contamination. ANAEROBIC CONDITIONS:Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in an anaerobic chamberLoose screw caps on test tubes in an activated anaerobic gas pack jarUse of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained Handling notesAnaerobe Systems Chopped Meat broth is recommended for growth. Brucella blood agar may be used in place of TSA+5% SB agar.Additional information on this culture is availableQuality control specificationsVerification methodWhole-genome SequencingHistoryDeposited asBacteroides putredinis (Weinberg et al.) KellyDepositorsEP CatoChain of custodyBioVector<-- EP Cato <-- S Suzuki 1 AndoType of isolateHumanSpecial collectionHuman Microbiome Project (HMP)Cross referencesGenBank L16497 Bacteroides putredinis ATCC 29800 16S ribosomal RNA gene, complete sequence.GenBank ABFK02000000 Alistipes putredinis DSM 17216, whole genome shotgun sequencing project
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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