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Alistipes putredinis strain另枝菌属菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49865
  • 货  号:Alistipes putredinis 
  • 产  地:北京
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Alistipes putredinis strain另枝菌属菌株

This is a type strain and a genome sequenced strain that has been sequenced as a reference genome for the NIH Human Microbiome Project.
Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI 3293 [1Ando, CCUG 45780, CIP 104286, DSM 17216]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Product format
Freeze-dried
BSL:2

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of Anaerobe Systems Chopped Meat broth. Aseptically transfer the entire contents to a 5-6 mL tube of Anaerobe Systems Chopped Meat broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 3-5 days. Incubate one agar plate aerobically at 37°C to check for contamination.


ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Handling notes
Anaerobe Systems Chopped Meat broth is recommended for growth. Brucella blood agar may be used in place of TSA+5% SB agar.
Additional information on this culture is available

Quality control specifications
Verification method
Whole-genome Sequencing
History
Deposited as
Bacteroides putredinis (Weinberg et al.) Kelly
Depositors
EP Cato
Chain of custody
BioVector<-- EP Cato <-- S Suzuki 1 Ando
Type of isolate
Human
Special collection
Human Microbiome Project (HMP)
Cross references
GenBank L16497 Bacteroides putredinis ATCC 29800 16S ribosomal RNA gene, complete sequence.
GenBank ABFK02000000 Alistipes putredinis DSM 17216, whole genome shotgun sequencing project

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