pCMV-LOx-IRES-EGFP plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCMV-LOx-IRES-EGFP plasmid vector质粒载体

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Vector backbonepEGFP-N1
(Search Vector Database)
Backbone manufacturerClontech
Backbone size w/o insert (bp)5078
Total vector size (bp)6203
Vector typeMammalian Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameLactate oxidase
Alt nameLOx
SpeciesSynthetic
Insert Size (bp)1125
GenBank IDD50611.1
PromoterCMV
Tag / Fusion Protein
EGFP (C terminal on backbone)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBglII (not destroyed)
3′ cloning sitePstI (not destroyed)
5′ sequencing primerCMV forward: ATGGGCGGTAGGCGTGTA
3′ sequencing primerIRES reverse: CCAAGTCAGTGGCTGCAC

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net