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不同功能空载体部分list-BioVector NTCC保藏中心

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Empty Backbone for Microbial Experiment

  • E. coli

Catalog num.Clone nameFormRegistance (E. coli)CloningPromoterTag
AmpTetCmlacT7FLAGHisGST












RDB01911Lambda GEX5Phage

Restriction Enzyme


RDB06084pETEG5Plasmid

Restriction Enzyme



RDB06211pT7Tet1Plasmid

Restriction Enzyme


RDB06212pT7Tet6Plasmid

Restriction Enzyme


RDB07041pETHFFcPlasmid

Gateway®

RDB07050pETFPlasmid

Restriction Enzyme


RDB07051pT7FCamPlasmid

Restriction Enzyme


  • Yeast


  • Expression Vector Backbone of Saccharomyces cerevisiae

    Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins.
    Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
    Depositor: Dr. Hidekatsu Iha
    Reference: Biotechniques, 25, 936-938 (1998)., PMID 9863043.

    Catalog no.NameTagOriMarkerPromoterSize (kb)Usable sites
    RDB01947pAMH10Myccentromere-typeHIS3ADH14.56
    RDB01948pAMH20Myc2micronHIS3ADH15.36
    RDB01949pAML10Myccentromere-typeLEU2ADH15.16
    RDB01950pAML20Myc2micronLEU2ADH15.96
    RDB01951pAMT10Myccentromere-typeTRP1ADH14.35
    RDB01952pAMT20Myc2micronTRP1ADH15.15
    RDB01953pAMU10Myccentromere-typeURA3ADH14.66
    RDB01954pAMU20Myc2micronURA3ADH15.46
    RDB01955pGMH10Myccentromere-typeHIS3GAL1-104.86
    RDB01956pGMH20Myc2micronHIS3GAL1-105.66
    RDB01957pGML10Myccentromere-typeLEU2GAL1-105.46
    RDB01958pGML20Myc2micronLEU2GAL1-106.26
    RDB01959pGMT10Myccentromere-typeTRP1GAL1-104.65
    RDB01960pGMT20Myc2micronTRP1GAL1-105.45
    RDB01961pGMU10Myccentromere-typeURA3GAL1-104.96
    RDB01962pGMU20Myc2micronURA3GAL1-105.76

    Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein.
    Depositor: Dr. Hiroshi Takagi
    Reference: Genes Cells, 18 (6): 459-475 (2013)., PMID 23517290.

    Catalog no.NameRunning title
    RDB08380pRS416-PADH1-ccdB-yEGFP-TTEF1Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.
    RDB08386pRS416-PADH1-yEGFP-TTEF1Empty expression vector backbone of S. cerevisiae


List of S. pombe Vector Series

See also http://www.riken.jp/SPD/Plasmids.html

See also NCBI VECTORS

S. pombe expression vectors (Gateway compatible)

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

  • Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotechnol. 2006 Jul; 24(7):841-7.

  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06130pDUAL-HFF1c
pDUAL
His6-FLAG2
5'
nmt1
ura4+
leu1+
[sequence]
RDB06485pDUAL-HFF21c
pDUAL
His6-FLAG2
5'
tub1
ura4+
leu1+
[sequence]
RDB06486pDUAL-HFF31c
pDUAL
His6-FLAG2
5'
cam1
ura4+
leu1+
[sequence]
RDB06131pDUAL-HFF41c
pDUAL
His6-FLAG2
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06487pDUAL-HFF51c
pDUAL
His6-FLAG2
5'
tif51
ura4+
leu1+
[sequence]
RDB06488pDUAL-HFF61c
pDUAL
His6-FLAG2
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06132pDUAL-HFF81c
pDUAL
His6-FLAG2
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06133pDUAL-HFG1c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
leu1+
[sequence]
RDB06493pDUAL-HFG21c
pDUAL
His6-FLAG-GFP(S65C)
5'
tub1
ura4+
leu1+
[sequence]
RDB06494pDUAL-HFG31c
pDUAL
His6-FLAG-GFP(S65C)
5'
cam1
ura4+
leu1+
[sequence]
RDB06134pDUAL-HFG41c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06495pDUAL-HFG51c
pDUAL
His6-FLAG-GFP(S65C)
5'
tif51
ura4+
leu1+
[sequence]
RDB06496pDUAL-HFG61c
pDUAL
His6-FLAG-GFP(S65C)
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06135pDUAL-HFG81c
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06136pDUAL-HFC1c
pDUAL
His6-FLAG-CFP
5'
nmt1
ura4+
leu1+
[sequence]
RDB06137pDUAL-HFC41c
pDUAL
His6-FLAG-CFP
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06138pDUAL-HFC81c
pDUAL
His6-FLAG-CFP
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06139pDUAL-HFY1c
pDUAL
His6-FLAG-YFP
5'
nmt1
ura4+
leu1+
[sequence]
RDB06140pDUAL-HFY41c
pDUAL
His6-FLAG-YFP
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06141pDUAL-HFY81c
pDUAL
His6-FLAG-YFP
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06142pDUAL-FFH1c
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06489pDUAL-FFH21c
pDUAL
FLAG2-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06490pDUAL-FFH31c
pDUAL
FLAG2-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06143pDUAL-FFH41c
pDUAL
FLAG2-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06491pDUAL-FFH51c
pDUAL
FLAG2-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06492pDUAL-FFH61c
pDUAL
FLAG2-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06144pDUAL-FFH81c
pDUAL
FLAG2-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06145pDUAL-GFH1c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06497pDUAL-GFH21c
pDUAL
GFP(S65C)-FLAG-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06498pDUAL-GFH31c
pDUAL
GFP(S65C)-FLAG-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06146pDUAL-GFH41c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06499pDUAL-GFH51c
pDUAL
GFP(S65C)-FLAG-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06500pDUAL-GFH61c
pDUAL
GFP(S65C)-FLAG-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06147pDUAL-GFH81c
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06148pDUAL-CFH1c
pDUAL
CFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06149pDUAL-CFH41c
pDUAL
CFP-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06150pDUAL-CFH81c
pDUAL
CFP-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06151pDUAL-YFH1c
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06152pDUAL-YFH41c
pDUAL
YFP-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06153pDUAL-YFH81c
pDUAL
YFP-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06481pDUAL-FFH1c rfA
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06482pDUAL-FFH1c rfC
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06483pDUAL-YFH1c rfA
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06484pDUAL-YFH1c rfC
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL2 series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06154pDUAL2-HFF1c
pDUAL2
His6-FLAG2
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06155pDUAL2-HFF41c
pDUAL2
His6-FLAG2
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06156pDUAL2-HFF81c
pDUAL2
His6-FLAG2
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06157pDUAL2-HFG1c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06158pDUAL2-HFG41c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06159pDUAL2-HFG81c
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06160pDUAL2-HFC1c
pDUAL2
His6-FLAG-CFP
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06161pDUAL2-HFC41c
pDUAL2
His6-FLAG-CFP
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06162pDUAL2-HFC81c
pDUAL2
His6-FLAG-CFP
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06163pDUAL2-HFY1c
pDUAL2
His6-FLAG-YFP
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06164pDUAL2-HFY41c
pDUAL2
His6-FLAG-YFP
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06165pDUAL2-HFY81c
pDUAL2
His6-FLAG-YFP
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06166pDUAL2-FFH1c
pDUAL2
FLAG2-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06167pDUAL2-FFH41c
pDUAL2
FLAG2-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06168pDUAL2-FFH81c
pDUAL2
FLAG2-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06169pDUAL2-GFH1c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06170pDUAL2-GFH41c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06171pDUAL2-GFH81c
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06172pDUAL2-CFH1c
pDUAL2
CFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06173pDUAL2-CFH41c
pDUAL2
CFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06174pDUAL2-CFH81c
pDUAL2
CFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06175pDUAL2-YFH1c
pDUAL2
YFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06503pDUAL2-YFH21c
pDUAL2
YFP-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06176pDUAL2-YFH41c
pDUAL2
YFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06177pDUAL2-YFH81c
pDUAL2
YFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06501pDUAL2-BFH41c
pDUAL2
BFP-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06502pDUAL2-BFH81c
pDUAL2
BFP-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at Chemical Genetics Laboratory.

pREP series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker

RDB06519pREP1-ccdB2
pREP1
-
-
nmt1
LEU2
[sequence]
RDB06520pREP41-ccdB2
pREP41
-
-
nmt1*
LEU2
[sequence]
RDB06521pREP81-ccdB2
pREP81
-
-
nmt1**
LEU2
[sequence]
RDB06522pHFF1-ccdB2
pREP1
His6-FLAG2
5'
nmt1
LEU2
[sequence]
RDB06523pHFF41-ccdB2
pREP41
His6-FLAG2
5'
nmt1*
LEU2
[sequence]
RDB06524pHFF81-ccdB2
pREP81
His6-FLAG2
5'
nmt1**
LEU2
[sequence]
RDB06525pHFG1-ccdB2
pREP1
His6-FLAG-GFP(S65C)
5'
nmt1
LEU2
[sequence]
RDB06526pHFG41-ccdB2
pREP41
His6-FLAG-GFP(S65C)
5'
nmt1*
LEU2
[sequence]
RDB06527pHFG81-ccdB2
pREP81
His6-FLAG-GFP(S65C)
5'
nmt1**
LEU2
[sequence]
RDB06506pFFH1-ccdB2
pREP1
FLAG2-His6
3'
nmt1
LEU2
[sequence]
RDB06507pFFH41-ccdB2
pREP41
FLAG2-His6
3'
nmt1*
LEU2
[sequence]
RDB06508pFFH81-ccdB2
pREP81
FLAG2-His6
3'
nmt1**
LEU2
[sequence]
RDB06509pGFH1-ccdB2
pREP1
GFP(S65C)-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06510pGFH41-ccdB2
pREP41
GFP(S65C)-FLAG-His6
3'
nmt1*
LEU2
[sequence]
RDB06511pGFH81-ccdB2
pREP81
GFP(S65C)-FLAG-His6
3'
nmt1**
LEU2
[sequence]
RDB06513pHFY1-ccdB2
pREP1
His6-FLAG-YFP
5'
nmt1
LEU2
[sequence]
RDB06512pHFC1-ccdB2
pREP1
His6-FLAG-CFP
5'
nmt1
LEU2
[sequence]
RDB06515pYFH1-ccdB2
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06505pYFH1-ccdB2 rfA
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06504pYFH1-ccdB2 rfC
pREP1
YFP-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06514pCFH1-ccdB2
pREP1
CFP-FLAG-His6
3'
nmt1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at Chemical Genetics Laboratory.

Gateway-compatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Tag
Tag
position
Multicopy marker
Targeted locus
(negative) selection
Positive selection marker

RDB06462pLYS1U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06463pLYS1U-CFH21c
pUC119
tub1
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06464pLYS1U-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06465pLYS1U-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
lys1+
ura4+
[sequence]
RDB06466pLYS1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06467pLYS1U-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06468pLYS1U-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06477pLYS1U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06478pLYS1U-YFH21c
pUC119
tub1
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06479pLYS1U-YFH61c
pUC119
ef1a-c
YFP-FLAG-His6
3'
-
lys1+
ura4+
[sequence]
RDB06469pLYS1U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06470pLYS1U-HFC21c
pUC119
tub1
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06471pLYS1U-HFC61c
pUC119
ef1a-c
His6-FLAG-CFP
5'
-
lys1+
ura4+
[sequence]
RDB06472pLYS1U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
lys1+
ura4+
[sequence]
RDB06473pLYS1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
lys1+
ura4+
[sequence]
RDB06474pLYS1U-HFY1c
pUC119
nmt1
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06475pLYS1U-HFY21c
pUC119
tub1
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06476pLYS1U-HFY61c
pUC119
ef1a-c
His6-FLAG-YFP
5'
-
lys1+
ura4+
[sequence]
RDB06458pLYS1K-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
lys1+
kanr
[sequence]
RDB06459pLYS1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
lys1+
kanr
[sequence]
RDB06460pLYS1K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
lys1+
kanr
[sequence]
RDB06461pLYS1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
lys1+
kanr
[sequence]
RDB06517pHIS3U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06455pHIS3U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06518pHIS3U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3'
-
his3+
ura4+
[sequence]
RDB06456pHIS3U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
his3+
ura4+
[sequence]
RDB06516pHIS3U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
his3+
ura4+
[sequence]
RDB06457pHIS3U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
his3+
ura4+
[sequence]
RDB06445pHIS3K-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06446pHIS3K-CFH21c
pUC119
tub1
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06447pHIS3K-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06448pHIS3K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06449pHIS3K-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06450pHIS3K-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3'
-
his3+
kanr
[sequence]
RDB06451pHIS3K-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5'
-
his3+
kanr
[sequence]
RDB06452pHIS3K-HFC21c
pUC119
tub1
His6-FLAG-CFP
5'
-
his3+
kanr
[sequence]
RDB06453pHIS3K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
his3+
kanr
[sequence]
RDB06454pHIS3K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
his3+
kanr
[sequence]
RDB06441pARG1U-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
arg1+
ura4+
[sequence]
RDB06442pARG1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
arg1+
ura4+
[sequence]
RDB06443pARG1U-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
arg1+
ura4+
[sequence]
RDB06444pARG1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
arg1+
ura4+
[sequence]
RDB06437pARG1K-FFH1c
pUC119
nmt1
FLAG2-His6
3'
-
arg1+
kanr
[sequence]
RDB06438pARG1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3'
-
arg1+
kanr
[sequence]
RDB06439pARG1K-HFF1c
pUC119
nmt1
His6-FLAG2
5'
-
arg1+
kanr
[sequence]
RDB06440pARG1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5'
-
arg1+
kanr
[sequence]
RDB06480pARG1KF-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3'
ura4+
arg1+
kanr
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

S. pombe expression vectors

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.

  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.

  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06178pDUAL
(pUC119)
-
-
-
ura4+
leu1+
[sequence]
RDB06528pDUAL-S
(pUC119)
-
-
-
ura4+
leu1+
[sequence]
RDB06529pDUAL-Pef1a
pDUAL
-
-
ef1a-c
ura4+
leu1+
[sequence]
RDB06530pDUAL-Ptub1
pDUAL
-
-
tub1
ura4+
leu1+
[sequence]
RDB06179pDUAL-HFF1
pDUAL
His6-FLAG2
5'
nmt1
ura4+
leu1+
[sequence]
RDB06531pDUAL-HFF21
pDUAL
His6-FLAG2
5'
tub1
ura4+
leu1+
[sequence]
RDB06532pDUAL-HFF31
pDUAL
His6-FLAG2
5'
cam1
ura4+
leu1+
[sequence]
RDB06180pDUAL-HFF41
pDUAL
His6-FLAG2
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06533pDUAL-HFF51
pDUAL
His6-FLAG2
5'
tif51
ura4+
leu1+
[sequence]
RDB06534pDUAL-HFF61
pDUAL
His6-FLAG2
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06181pDUAL-HFF81
pDUAL
His6-FLAG2
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06182pDUAL-HFG1
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
leu1+
[sequence]
RDB06539pDUAL-HFG21
pDUAL
His6-FLAG-GFP(S65C)
5'
tub1
ura4+
leu1+
[sequence]
RDB06540pDUAL-HFG31
pDUAL
His6-FLAG-GFP(S65C)
5'
cam1
ura4+
leu1+
[sequence]
RDB06183pDUAL-HFG41
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
leu1+
[sequence]
RDB06541pDUAL-HFG51
pDUAL
His6-FLAG-GFP(S65C)
5'
tif51
ura4+
leu1+
[sequence]
RDB06542pDUAL-HFG61
pDUAL
His6-FLAG-GFP(S65C)
5'
ef1a-c
ura4+
leu1+
[sequence]
RDB06184pDUAL-HFG81
pDUAL
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
leu1+
[sequence]
RDB06185pDUAL-FFH1
pDUAL
FLAG2-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06535pDUAL-FFH21
pDUAL
FLAG2-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06536pDUAL-FFH31
pDUAL
FLAG2-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06186pDUAL-FFH41
pDUAL
FLAG2-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06537pDUAL-FFH51
pDUAL
FLAG2-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06538pDUAL-FFH61
pDUAL
FLAG2-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06187pDUAL-FFH81
pDUAL
FLAG2-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06188pDUAL-GFH1
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]
RDB06543pDUAL-GFH21
pDUAL
GFP(S65C)-FLAG-His6
3'
tub1
ura4+
leu1+
[sequence]
RDB06544pDUAL-GFH31
pDUAL
GFP(S65C)-FLAG-His6
3'
cam1
ura4+
leu1+
[sequence]
RDB06189pDUAL-GFH41
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
leu1+
[sequence]
RDB06545pDUAL-GFH51
pDUAL
GFP(S65C)-FLAG-His6
3'
tif51
ura4+
leu1+
[sequence]
RDB06546pDUAL-GFH61
pDUAL
GFP(S65C)-FLAG-His6
3'
ef1a-c
ura4+
leu1+
[sequence]
RDB06190pDUAL-GFH81
pDUAL
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
leu1+
[sequence]
RDB06191pDUAL-YFH1
pDUAL
YFP-FLAG-His6
3'
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at Chemical Genetics Laboratory.

pDUAL2 series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Multicopy marker
Integration marker

RDB06192pDUAL2
(pUC119)
-
-
-
ura4+
ura4+, leu1-
[sequence]
RDB06193pDUAL2-HFF1
pDUAL2
His6-FLAG2
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06547pDUAL2-HFF21
pDUAL2
His6-FLAG2
5'
tub1
ura4+
ura4+, leu1-
[sequence]
RDB06194pDUAL2-HFF41
pDUAL2
His6-FLAG2
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06549pDUAL2-HFF61
pDUAL2
His6-FLAG2
5'
ef1a-c
ura4+
ura4+, leu1-
[sequence]
RDB06195pDUAL2-HFF81
pDUAL2
His6-FLAG2
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06196pDUAL2-HFG1
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06197pDUAL2-HFG41
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06198pDUAL2-HFG81
pDUAL2
His6-FLAG-GFP(S65C)
5'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06199pDUAL2-FFH1
pDUAL2
FLAG2-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06548pDUAL2-FFH21
pDUAL2
FLAG2-His6
3'
tub1
ura4+
ura4+, leu1-
[sequence]
RDB06200pDUAL2-FFH41
pDUAL2
FLAG2-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06550pDUAL2-FFH61
pDUAL2
FLAG2-His6
3'
ef1a-c
ura4+
ura4+, leu1-
[sequence]
RDB06201pDUAL2-FFH81
pDUAL2
FLAG2-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06202pDUAL2-GFH1
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1
ura4+
ura4+, leu1-
[sequence]
RDB06203pDUAL2-GFH41
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1*
ura4+
ura4+, leu1-
[sequence]
RDB06204pDUAL2-GFH81
pDUAL2
GFP(S65C)-FLAG-His6
3'
nmt1**
ura4+
ura4+, leu1-
[sequence]
RDB06551pDUAL2-CFH61
pDUAL2
CFP-FLAG-His6
3'
ef1a-c
ura4+
ura4+, leu1-
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at Chemical Genetics Laboratory.

pREP series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker

RDB06580pHFF1
pREP1
His6-FLAG2
5'
nmt1
LEU2
[sequence]
RDB06581pHFF41
pREP41
His6-FLAG2
5'
nmt1*
LEU2
[sequence]
RDB06582pHFF81
pREP81
His6-FLAG2
5'
nmt1**
LEU2
[sequence]
RDB06592pHFF148
(pREP)
His6-FLAG2
5'
ADH1
LEU2
[sequence]
RDB06583pHFG1
pREP1
His6-FLAG-GFP(S65C)
5'
nmt1
LEU2
[sequence]
RDB06584pHFG41
pREP41
His6-FLAG-GFP(S65C)
5'
nmt1*
LEU2
[sequence]
RDB06585pHFG81
pREP81
His6-FLAG-GFP(S65C)
5'
nmt1**
LEU2
[sequence]
RDB06593pHFG148
(pREP)
His6-FLAG-GFP(S65C)
5'
ADH1
LEU2
[sequence]
RDB06586pFFH1
pREP1
FLAG2-His6
3'
nmt1
LEU2
[sequence]
RDB06587pFFH41
pREP41
FLAG2-His6
3'
nmt1*
LEU2
[sequence]
RDB06588pFFH81
pREP81
FLAG2-His6
3'
nmt1**
LEU2
[sequence]
RDB06594pFFH148
(pREP)
FLAG2-His6
3'
ADH1
LEU2
[sequence]
RDB06589pGFH1
pREP1
GFP(S65C)-FLAG-His6
3'
nmt1
LEU2
[sequence]
RDB06590pGFH41
pREP41
GFP(S65C)-FLAG-His6
3'
nmt1*
LEU2
[sequence]
RDB06591pGFH81
pREP81
GFP(S65C)-FLAG-His6
3'
nmt1**
LEU2
[sequence]
RDB06595pGFH148
(pREP)
GFP(S65C)-FLAG-His6
3'
ADH1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at Chemical Genetics Laboratory.

Gateway-incompatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Multicopy marker
Targeted locus
Positive selection marker

RDB06552pLYS1U
pUC119
-
-
lys1+
ura4+
[sequence]
RDB06567pLYS1U-Pnmt1
pUC119
nmt1
-
lys1+
ura4+
[sequence]
RDB06566pLYS1U-Ptub1
pUC119
tub1
-
lys1+
ura4+
[sequence]
RDB06553pLYS1K
pUC119
-
-
lys1+
kanr
[sequence]
RDB06554pLYS1H
pUC119
-
-
lys1+
hygr
[sequence]
RDB06555pLYS1B
pUC119
-
-
lys1+
blar
[sequence]
RDB06560pHIS3U
pUC119
-
-
his3+
ura4+
[sequence]
RDB06565pHIS3U-Pnmt1
pUC119
nmt1
-
his3+
ura4+
[sequence]
RDB06561pHIS3K
pUC119
-
-
his3+
kanr
[sequence]
RDB06564pHIS3K-Ptub1
pUC119
tub1
-
his3+
kanr
[sequence]
RDB06562pHIS3H
pUC119
-
-
his3+
hygr
[sequence]
RDB06563pHIS3B
pUC119
-
-
his3+
blar
[sequence]
RDB06556pARG1U
pUC119
-
-
arg1+
ura4+
[sequence]
RDB06557pARG1K
pUC119
-
-
arg1+
kanr
[sequence]
RDB06558pARG1H
pUC119
-
-
arg1+
hygr
[sequence]
RDB06559pARG1B
pUC119
-
-
arg1+
blar
[sequence]
RDB06573pLYS1UF
pUC119
-
ura4+
lys1+
ura4+
[sequence]
RDB06572pLYS1KF
pUC119
-
ura4+
lys1+
kanr
[sequence]
RDB06574pLYS1HF
pUC119
-
ura4+
lys1+
hygr
[sequence]
RDB06575pLYS1BF
pUC119
-
ura4+
lys1+
blar
[sequence]
RDB06569pHIS3UF
pUC119
-
ura4+
his3+
ura4+
[sequence]
RDB06568pHIS3KF
pUC119
-
ura4+
his3+
kanr
[sequence]
RDB06570pHIS3HF
pUC119
-
ura4+
his3+
hygr
[sequence]
RDB06571pHIS3BF
pUC119
-
ura4+
his3+
blar
[sequence]
RDB06577pARG1UF
pUC119
-
ura4+
arg1+
ura4+
[sequence]
RDB06576pARG1KF
pUC119
-
ura4+
arg1+
kanr
[sequence]
RDB06578pARG1HF
pUC119
-
ura4+
arg1+
hygr
[sequence]
RDB06579pARG1BF
pUC119
-
ura4+
arg1+
blar
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter


Expression Vector Backbone of Schizosaccharomyces pombe

Depositor: Dr. Toshiaki Isobe
Reference: PLoS One 9, e112488 (2014)., PMID 25401760.

Catalog No.plasmidbackborneSp markerEc markerinsertremarks
RDB08041pCtrlki-kanMX6bacterialkanMX6ApMCS1-opal stop codon-kanMX6-MCS2Plasmid vector to constract C-terminal truncated allele by the integration of opal stop codon into target gene using homologous recombination.
RDB08042pCtFEM3ki-kanMX6bacterialkanMX6ApMCS1-Flag-tev-3myc-opal stop codon-kanMX6-MCS2Plasmid vector to construct C-terminally FEM3 (FLAG-tev-3xmyc) tagged allele of target gene by using homologous recombination.
RDB08045pCtFLATAki-kanMX6bacterialkanMX6ApMCS1-Flag-tev-protein A-opal stop codon-kanMX6-MCS2Plasmid vector to construct C-terminally FLATA (FLAG-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08048pFOX1-CFLATAreplicativeScLEU2(complement leu1-)ApPtef-MCS1-Flag-tev-protein A-opal stop codon-TtefPlasmid vector to constract replicative expression clone constitutively expressing C-terminally FLATA tag (FLAG-tev-protein A) allele in leu1- strain of S. pombe.
RDB08051pCtHATAki-kanMX6bacterialkanMX6ApMCS1-HA-tev-protein A-opal stop codon-kanMX6-MCS2Plasmid vector to constract C-terminally HATA (HA-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08052pFOX1-CHATAreplicativeScLEU2(complement leu1-)ApPtef-MCS1-HA-tev-protein A-opal stop codon-TtefPlasmid vector to constract replicative expression clone constitutively expressing C-terminally HATA tag (HA-tev-protein A) allele in leu1- strain of S. pombe.

  • Bacillus

  • RDB00919pSTE12Bacillus stearothermophilus shuttle vector
    RDB00920pSTE33Bacillus stearothermophilus shuttle vector

Gene Disruption


Empty Backbone for Mammalian Cell Experiment

  • Plasmid/cosmid


Catalog num.Clone NameEpitope TagSelectable MarkerCloningPromoterBacterial Resistance
FlagHA2xHAT72xT7Myc2xMycw/oNeow/oRestriction EnzymeGateway®CMVCAGEF-1RSVAmp
RDB05956pCMV_S-FLAG











RDB06083pRSV_S-FLAG











RDB07396pCMV_S-FLAGc











RDB07397pRSV_s-FLAGc











RDB02136S-HA-pRc/CMV











RDB02139D-HA-pRc/CMV











RDB02137S-T7-pRc/CMV











RDB02138D-T7-pRc/CMV











RDB02140S-Myc-pRc/CMV











RDB02141D-Myc-pRc/CMV











RDB07939pEF-BOS











RDB07940pEF-BOS-EX











RDB08938pCAGGS































    RDBName of cloneRunning titleClassSeq file
    1379pH2RneoExpression vector with simian virus 40 (SV40) early promoterVector
    1674pCALNLwA cassette to introduce cDNA into mammalian chromosomeVector
    1679pCALwLA cassette plasmid to insert cDNA into downstream CAG promoterVector
    1862pCALNL5A cassette to introduce cDNA into mammalian chromosomeVector
    1968pBMSAExpression vector, inducible promoterVector
    2546pxCAGExpression vector driven by CAG promoterVector
    3043pMNSMRetrovirus expression vector in eukaryotic cellsVector

pCMV_tag and pRSV_tag assay-ready clones


Vectors (ja)
Vectors (en)
Insert sequences
FAQs

Laboratory use only.
Not intended for intentional introduction into the environment.


RDBName of cloneRunning titleClassCloning siteSeq file
2136S-HA-pRc/CMVExpressing HA-tagged fusion protein, CMV promoterVectorRDB2136.pdfRDB2136z.seq
2139D-HA-pRc/CMVExpressing a fusion protein tagged with duplicated HA, CMV promoterVectorRDB2139.pdfRDB2139z.seq
2137S-T7-pRc/CMVExpressing T7-tagged fusion protein, CMV promoterVectorRDB2137.pdfRDB2137z.seq
2138D-T7-pRc/CMVExpressing a fusion protein tagged with duplicated T7, CMV promoterVectorRDB2138.pdfRDB2138z.seq
2140S-Myc-pRc/CMVExpressing Myc-tagged fusion protein, CMV promoterVectorRDB2140.pdfRDB2140z.seq
2141D-Myc-pRc/CMVExpressing a fusion protein tagged with duplicated Myc epitopes, CMV promoterVectorRDB2141.pdfRDB2141z.seq

Expression vectors listed above are constructed by Dr. Yoshihiro Takemoto of Tokyo Medical and Dental University and published in the DNA Cell Biol. 16 (7): 893-896, 1997. You can select one or two copies of epitope tags among the HA, T7 and Myc. The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest with these vectors. After the cloning of your gene, the NotI site also allows inserted gene to be moved to another vectors among these 6 vectors.
Please visit the following site for genetic materials deposited to our division by Dr. Takemoto.
http://dna.brc.riken.jp/search/dep3357.html

go to Head

RDBName of cloneRunning titleClassCloning siteSeq file
6083pRSV_S-FLAGExpressing FLAG-tagged fusion protein, RSV promoterVectorRDB6083.pdfRDB6083z.seq

go to Head

RDBName of cloneRunning titleClassCloning siteSeq file
5956pCMV_S-FLAGExpressing FLAG-tagged fusion protein, CMV promoterVectorRDB5956.pdfRDB5956z.seq
6072pCMFlag_lacZExpression vector of lacZ, with FLAG-tag, control vectorDNA cloneRDB6072.pdf
6071pCMFlag_EGFPExpression vector of GFP, with FLAG-tag, control vectorDNA cloneRDB6072.pdf

pCMV_S-FLAG vector is a mammalian expression vector designed to express proteins with FLAG epitope tag (Met-DYKDDDDK). The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest. The first ATG of the FLAG tag is included in the NcoI restriction enzyme site (CCATGG) and the NcoI site is available for cloning in-frame the FLAG tag and your interested gene together into bacterial expression vectors. The EcoRI site abutting upon the FLAG tag allows to excite your interested gene with the FLAG tag. PCR products starting with ATG codon can be cloned in-flame into the blunted NotI restriction site.




RDBName of cloneRunning titleClassCloning siteSeq file
7396pCMV_s-FLAGcDestination vector to generate eukaryotic expression vector, with FLAG-tagVectorRDB7396.pdfRDB7396z.seq
pCMV_s-FLAGc vector is a derivative of the pCMV_S-FLAG vector. Gateway® technology offers one-step cloning of your interested gene in Entry clones into the pCMV_s-FLAGc expression vector. Please refer the following website for Entry clones from the Genome Network Project Human Full-Length cDNA.
http://dna.brc.riken.jp/en/GNPcloneen.html

Empty Backbone for Recombinant Virus

  • Adenovirus


Empty vectors for construction of recombinant adenovirus


RDB no.Name of vectorShort titleClassification
917pAxcwA cassette cosmid for construction of recombinant adenovirusVector
918pAxcA cassette cosmid for construction of recombinant adenovirusVector
1678pAxCAwtA cassette cosmid for construction of a recombinant adenovirusVector
2564pAxCEAwtRCloning vector to generate recombinant adenovirus driven by human CEA promoterVector
2565pAxCEAwtCloning vector to generate recombinant adenovirus driven by human CEA promoterVector
3120pAxcwitA dual cassette for constructing recombinant adenovirusVector
3121pAxCAwtitA dual cassette for constructing recombinant adenovirus containing CAG promoterVector
3220pAF16RctCosmid clone of genomic fragment of adenovirus type 5Vector
3380pAxCAiLacZitShuttle vector to generate recombinant adenovirus harbouring E. coli LacZShuttleVector
3444pUAd5LShuttle vector to generate recombinant adenovirusVector
3445pUAd5LCAShuttle vector to generate recombinant adenovirusVector
3446pUAd5LCALNLShuttle vector to generate recombinant adenovirusVector
3447pUAd5LCAShuttle vector to generate recombinant adenovirusVector
3448pUAd5LCALNLShuttle vector to generate recombinant adenovirusVector
5212pAxcwit2A dual cassette for constructing recombinant adenovirusVector
5213pAxCAwtit2A dual cassette for constructing recombinant adenovirusVector
5214pAxCALNLwtit2A dual cassette for constructing recombinant adenovirusVector
5215pAxEFwtit2A dual cassette for constructing recombinant adenovirusVector


Name of vectorCloning sitePromoteraCre-loxPbProduction of recombinant adenovirusRDB no.Map
Intact-genome transfectioncTwo-cosmiddCOS-TPCeFiber-modified
COS-TPC
pAxcwSwaI



Yes
917[ pdf ]
pAxCAwtSwaICA


Yes
1678[ pdf ]
pAxCALNLwSwaICAYes

Yes
f
pAxcwitSwaI

Yes
Yes
3120[ pdf ]
pAxCAwtitSwaICA
Yes
Yes
3121[ pdf ]
pUAd5LSwaI


YesYesYes3444[ pdf ]
pUAd5LCASwaICA

YesYesYes3445[ pdf ]
pUAd5LCALNLSwaICAYes
YesYesYes3446[ pdf ]
pAxCEAwtSwaICEA


Yes
2565[ pdf ]
pAxcwit2SwaI

Yesg
Yes
5212[ pdf ]
pAxCAwtit2SwaICA
Yesg
Yes
5213[ pdf ]
pAxCALNLwtit2SwaICAYesYesg
Yes
5214[ pdf ]
pAxEFwtit2SwaIEF-1 alpha
Yesg
Yes
5215[ pdf ]

a, CA, a modified promoter of the chicken gene for beta-actin with a cytomegalovirus-immediate early enhancer (CMV-IE); CEA, carcinoembryonic antigen; EF-1 alpha, elongation factor 1 alpha.

b, Target gene harbored in the recombinant adenovirus can be expressed by co-infection with AxCANCre (RDB no. 1748, Gene 181: 207-212, 1996).

c, Fukuda et al., Microbiol. Immunol. 50: 643-654, 2006.

d, Use pAF16Rct (RDB no. 3220) together to generate rAd.

e, Miyake et al., Proc. Natl. Acad. Sci. USA 93: 1320-1324, 1996.

f, Available from Takara Bio Inc.

g, PacI restriction enzyme is also applicable for linearization as well as Csp45I restriction enzyme.



Vectors to construct shuttle vector for recombinant adenovirus.
Pdf file is available.



  • Retrovirus


  • RDB no.Name of vectorShort titleClassification
    1699pRx nZ ires NeoNls (nuclear localization signal), lacZ, ires, neoVector


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