pOET1.1N 6xHis plasmid vector杆状病毒表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：pOET1.1N 6xHis
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pOET1.1N 6xHis plasmid vector杆状病毒表达质粒载体
pOET1.1N 6xHis is a baculovirus transfer vector designed for high level expression of foreign genes under the powerfulAcMNPV polyhedrin (polh) promoter (Pr_PH). The vector encodes an optional N-terminal 6xHis-Tag® fusion sequence that maybe utilised if the insert allows read-through in the correct reading frame. This greatly eases the purification of the recombinantprotein since the 6xHis-containing fusion proteins bind with high affinity to Ni-NTA Agarose. If required, the 6xHis-Tag® canbe removed by incubating the fusion protein in the presence of the proteinase cleavage enzyme Thrombin. pOET1.1N is smallerthan other available transfer vectors (4625 bp) which greatly facilitates the cloning steps. It has a Col E1 origin of replicationand an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS)containing unique restriction enzyme sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequencesflanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into thepolh locus. pOET1.1N is compatible with any baculovirus system that utilises homologous recombination in insect cells.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
pOET1.1N 6xHis is a baculovirus transfer vector designed for high level expression of foreign genes under the powerfulAcMNPV polyhedrin (polh) promoter (Pr_PH). The vector encodes an optional N-terminal 6xHis-Tag® fusion sequence that maybe utilised if the insert allows read-through in the correct reading frame. This greatly eases the purification of the recombinantprotein since the 6xHis-containing fusion proteins bind with high affinity to Ni-NTA Agarose. If required, the 6xHis-Tag® canbe removed by incubating the fusion protein in the presence of the proteinase cleavage enzyme Thrombin. pOET1.1N is smallerthan other available transfer vectors (4625 bp) which greatly facilitates the cloning steps. It has a Col E1 origin of replicationand an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS)containing unique restriction enzyme sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequencesflanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into thepolh locus. pOET1.1N is compatible with any baculovirus system that utilises homologous recombination in insect cells.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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