Parabacteroides goldsteinii strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Parabacteroides goldsteinii strain菌株

Parabacteroides goldsteinii
BioVector-BAA-1180

Type strain
Product category
Bacteria
Product type
Anaerobe
Strain designation
WAL 12034, CCUG 48944
Type strain
Yes
Isolation source
Tissue
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Biosafety Level 2

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.
Under anaerobic conditions, withdraw 0.5 mL of #1490 broth from a single test tube (5 to 6 mL) and rehydrate the entire vial contents.
Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 mL each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
Incubate tubes and one plate under anaerobic conditions at 37°C. Incubate one blood plate aerobically at 37°C.
Within 48 hours colonies on the anaerobic blood plate are pinpoint, circular, entire, convex, and translucent. No growth should occur on the aerobic plate.


ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber, or
Placement of test tubes under a gassing cannula system hooked to anaerobic gas.


Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber,
Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

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