三叶草根瘤菌TA-1 BioVector NTCC质粒载体菌种细胞基因保藏中心

三叶草根瘤菌TA-1

Rhizobium trifolii TA1 and Rhizobium leguminosarum MNF 3841 grow on a range of aromatic substrates. R. trifolii TA1 possesses enzymes of both the catechol and protocatechuate pathways, whereas R. leguminosarum MNF 3841 only has enzymes of the latter pathway. The pathways are induced by growth on benzoate or 4-hydroxybenzoate, respectively, but they are not cross-inducible. 4-Hydroxybenzoate permease and hydroxylase are induced by growth on 4-hydroxybenzoate but not on protocatechuate, suggesting that they are regulated separately from protocatechuate dioxygenase. The uptake systems for both benzoate and 4-hydroxybenzoate are inhibited by azide, carbonyl cyanide m-chlorophenyl hydrazone and N, N′-dicyclohexylcarbodiimide but are insensitive to arsenate. Salicylate and protocatechuate interfere with benzoate and 4-hydroxybenzoate uptake, respectively.

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