Lachnospira multipara strain多对毛螺菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98865
- 货 号：Lachnospira multipara
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Lachnospira multipara strain多对毛螺菌菌株
Lachnospira multipara Bryant and Small19207Product categoryBacteriaProduct typeAnaerobeStrain designation[ATCC 12563, D32, DSM 3073]Type strainYesIsolation sourceRumenBiosafety Icon BSL 1Medium 602: E medium for AnaerobesTemperature35-40°CAtmosphereAnaerobicHandling procedureImmediately prior to working with the culture, place the frozen vial at room temperature to thaw under a gentle stream of anaerobic gas. Alternatively, place the vial in an anaerobic glove box while it thaws.Under anaerobic conditions, withdraw the entire contents of the vial and transfer to a tube of recommended anaerobic broth. Mix well.A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.Incubate tubes and plate under anaerobic conditions at 35-40°C. Incubate blood plate aerobically at 37°C.Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.ANAEROBIC CONDITIONS: Anaerobic conditions for transfer may be obtained by either of the following: Use of an anaerobic gas chamber, or Placement of test tubes under a gassing cannula system hooked to anaerobic gas. Anaerobic conditions for incubation may be obtained by any of the following: Loose screw caps on test tubes in anaerobic chamber, Loose screw caps on test tubes in an activated anaerobic gas pack jar, or Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained. Handling notesAlways use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Lachnospira multipara Bryant and Small19207Product categoryBacteriaProduct typeAnaerobeStrain designation[ATCC 12563, D32, DSM 3073]Type strainYesIsolation sourceRumenBiosafety Icon BSL 1Medium 602: E medium for AnaerobesTemperature35-40°CAtmosphereAnaerobicHandling procedureImmediately prior to working with the culture, place the frozen vial at room temperature to thaw under a gentle stream of anaerobic gas. Alternatively, place the vial in an anaerobic glove box while it thaws.Under anaerobic conditions, withdraw the entire contents of the vial and transfer to a tube of recommended anaerobic broth. Mix well.A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.Incubate tubes and plate under anaerobic conditions at 35-40°C. Incubate blood plate aerobically at 37°C.Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.ANAEROBIC CONDITIONS: Anaerobic conditions for transfer may be obtained by either of the following: Use of an anaerobic gas chamber, or Placement of test tubes under a gassing cannula system hooked to anaerobic gas. Anaerobic conditions for incubation may be obtained by any of the following: Loose screw caps on test tubes in anaerobic chamber, Loose screw caps on test tubes in an activated anaerobic gas pack jar, or Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained. Handling notesAlways use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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