Bdellovibrio bacteriovorus HD 100 strain蛭弧菌菌株 (ATCC 15356) BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Bdellovibrio bacteriovorus HD 100 (ATCC 15356)
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Bdellovibrio bacteriovorus HD 100 strain蛭弧菌菌株 (ATCC 15356)
Bdellovibrio bacteriovorus Stolp and Starr15356™Type strainProduct categoryBacteriaStrain designation100 [NCIB 9529]Type strainYesGenome sequenced strainYesIsolation sourceSoilGeographical isolationUnited States; CaliforniaProduct formatFreeze-driedStorage conditions2°C to 8°CCharacteristicsCommentsDistributed in the plant pathogen Erwinia amylovora ATCC 15357Genome sequenced strainHandling informationMediumATCC Medium 1603: DNB mediumTemperature30°CAtmosphereAerobicHandling procedureOpen vial according to the enclosed instructions.Using a 125 mL flask containing 50 mL of the recommended broth, withdraw 0.5 to 1.0 mL and rehydrate the entire pellet. Put several drops into a tube of standard Nutrient Broth (BD 234000) and the rest back into the flask.Incubate the tube at 30oC and the flask on a reciprocal shaker at the same temperature.After 24 hours, place the tube in the refrigerator to be used as a host stock if needed. It will also contain the Bdellovibrio but often a more concentrated suspension of the host is needed for infection. A pure culture of the host strain ATCC® 15357™ Erwinia amylovora is available and may also be used instead of trying to propagate the host as a mixed culture.After 5-10 days, examine a wet mount preparation of the flask culture to check for free swimming Bdellovibrio cells. They will be observed as very tiny, actively motile, curved rods.Handling notesObserve the flask culture microscopically daily until the Bdellovibrio are observed. A decrease in turbidity should also be noted indicating lysis of the host cells.To further propagate the strain, use the tube of the host set aside for this purpose. It should be transferred and used as a 24 hour culture.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Bdellovibrio bacteriovorus Stolp and Starr15356™Type strainProduct categoryBacteriaStrain designation100 [NCIB 9529]Type strainYesGenome sequenced strainYesIsolation sourceSoilGeographical isolationUnited States; CaliforniaProduct formatFreeze-driedStorage conditions2°C to 8°CCharacteristicsCommentsDistributed in the plant pathogen Erwinia amylovora ATCC 15357Genome sequenced strainHandling informationMediumATCC Medium 1603: DNB mediumTemperature30°CAtmosphereAerobicHandling procedureOpen vial according to the enclosed instructions.Using a 125 mL flask containing 50 mL of the recommended broth, withdraw 0.5 to 1.0 mL and rehydrate the entire pellet. Put several drops into a tube of standard Nutrient Broth (BD 234000) and the rest back into the flask.Incubate the tube at 30oC and the flask on a reciprocal shaker at the same temperature.After 24 hours, place the tube in the refrigerator to be used as a host stock if needed. It will also contain the Bdellovibrio but often a more concentrated suspension of the host is needed for infection. A pure culture of the host strain ATCC® 15357™ Erwinia amylovora is available and may also be used instead of trying to propagate the host as a mixed culture.After 5-10 days, examine a wet mount preparation of the flask culture to check for free swimming Bdellovibrio cells. They will be observed as very tiny, actively motile, curved rods.Handling notesObserve the flask culture microscopically daily until the Bdellovibrio are observed. A decrease in turbidity should also be noted indicating lysis of the host cells.To further propagate the strain, use the tube of the host set aside for this purpose. It should be transferred and used as a 24 hour culture.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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