U-2932 Cell Line人弥漫大B细胞淋巴瘤细胞株/完全培养基-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥98965
- 货 号:U-2932
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
U2932 cell line人弥漫大B细胞淋巴瘤细胞株
Cell line name U-2932
Synonyms U2932
Cat No.: NTCC912431
Species validation:human (Homo sapiens)
Cell type:B cell lymphoma
Origin:established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available
NTCCCell Culture Data:
Morphology:small round cells growing singly and in clusters in suspension;
Image:
Medium:90% RPMI 1640 + 10% h.i. FBS
Subculture:seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:3 twice a week, maintain at 1-1.5 x 106 cells/ml; could be of advantage to start culture in a 12- or 24-well plate
Incubation:at 37 °C with 5% CO2
Doubling time:ca. 50 hours
Harvest:cell harvest of ca. 2 x 106 cells/ml
Storage:frozen with 70% medium, 20% FBS, 10% DMSO
NTCCScientific Data:
Mycoplasma:negative in microbiological culture, PCR assays
Immunology:CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -;
Authenticity:STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human polyclonal hypodiploid karyotype with 20% polyploidy; 45(43-46)<2n>, XX, add(X)(q22), add(1)(q24), der(3)ins(3;18)(q27;q2?q2?)hsr(18)(q21), add(5)(q32), der(6)t(6;18)(p24;?q23)del(6)(q13), der(10)t(10;14)(q24;q23), der(11)t(1;11)(q25;q2?), der(14)t(3;14)(q27;p11), der(18)t(1;18)(q21;q21), der(18)t(3;18)(q2?;q27), add(19)(p13), del(19)(q13); sdl with del(2)(q11), t(4;15)(q22;q14), add(7)(q21), del(13)(?q21), der(13)add(13)(p11)add(13)(q32); extensive genomic amplification of BCL2 region; resembles published karyotype
Viruses:PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
CommentsPart of: LL-100 blood cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Characteristics: Genetic heterogeneous, consists of 2 subclones (PubMed=27566572).
Doubling time: 48 hours (PubMed=12533045); ~50 hours.
Omics: Cell surface proteome.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Miscellaneous: Originally the patient was suffering from Hodgkin lymphoma.
Derived from sampling site: Ascites.
DiseaseDiffuse large B-cell lymphoma activated B-cell type (NCIt: C36081)
Diffuse large B-cell lymphoma (ORDO: Orphanet_544)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cellFemale
Age at sampling29Y
Category Cancer cell line
Name:U-2932 Cell Line
中文名称:
Organism种属:
Comments: DLBCL, young adult, terminal
Cell Type细胞类型:
Morphology形态:
Culture Properties培养特性:
Disease疾病:
Biosafety Level生物安全级别:
Age年龄:
Gender性别:
Applications应用:
Images图片:
Complete Growth Medium完全培养基:
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:
Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
http://www.biovector.net
- 公告/新闻