pmEosFP(Thermostab), FLAG-tagged BioVector NTCC质粒载体菌种细胞基因保藏中心

pmEosFP(Thermostab), FLAG-tagged

pmEosFP(Thermostab) with monomeric EosFP(Thermostab) and FLAG®
-tag.
Introduction
pmEosFP(Thermostab) is an advanced variant of the green-to-red photoconvertible fluorescent
protein EosFP1,2. The marker was optimized for functional expression at 37 °C, especially in fusion
with other proteins. pmEosFP(Thermostab) retains the monomeric nature of its predecessor and
shows an excellent performance in fusion even with demanding partners such as tubulin (Fig. 1).
pmEosFP(Thermostab) can be efficiently converted from green to red by a light pulse at wavelengths
between 360 and 430 nm. Nuclear localization of the recombinant signal-binding protein
pmEosFP(Thermostab) and tubulin-pmEosFP(Thermostab) is shown in Figure 1 (bottom left and
center). Subsequently, the protein of interest can be tracked by the red fluorescence of the
photoconverted form of pmEosFP(Thermostab). The marker protein can be also used to study cell
movement, e.g. during embryonic development3

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