pmIrisFP, FLAG-tagged BioVector NTCC质粒载体菌种细胞基因保藏中心

pmIrisFP, FLAG-tagged

pmIrisFP with monomeric IrisFP and FLAG®
-tag.
Introduction
pmIrisFP is a photoactivatable fluorescent protein that combines irreversible photo-conversion from a
green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Suitable laser lines for photoswitching are indicated in Fig. 1. pmIrisFP
is a monomeric variant of IrisFP with low tendency of dimer formation. Its multiple photoactivation
modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in
living cells by using dual-color photo-activation localization microscopy (PALM)1

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