pBHR1 Broad Host Range Vector (mobilizable)广宿主载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBHR1 Broad Host Range Vector (mobilizable)广宿主载体质粒

Broad-host-range vectors pBBR122 and pBHR1 suitable for
· cloning and gene expression in bacteria other than E. coli (mainly Gram-negative)
· changing bacterial hosts
· studies of broad-host-range replicons
· studies of Gram-negative bacteria
Description
pBBR122 broad-host-range vector, not mobilizable
The plasmid pBBR122 is a cloning vector with very broad-host-range maintenance properties. Unlike
other broad-host-range vectors, it replicates at medium copy numbers and has a small size (5307 bp).
This greatly facilitates genetic studies of a wide variety of Gram-negative bacteria and makes pBBR122
particularly interesting for studies of broad-host-range replicons. Being a versatile expression vector, it
stably replicates in all Gram-negative organisms tried so far under standard growth conditions (see list
below) using appropriate selective pressure.
pBBR122 carries a nonfunctional copy of the mobilization gene (mob’). Therefore it is neither mobilizable
nor conjugative. For cloning and selection there are two resistance genes placed within the vector, kan
(kanamycin resistance) and cm (chloramphenicol resistance). The rep gene is essential for replication.
pBBR122 was derived from pBBR1, which was isolated from Bordetella bronchiseptica S87 (Antoine &
Locht, 1992; Renauld-Mongénie et al., 1996). It is compatible with other broad-host-range vectors, since it
does not belong to the broad-host-range incompatibility groups IncP, IncQ or IncW.
pBHR1 broad-host-range vector, mobilizable
By removing a frame shift in the mob’ gene of pBBR122, the gene function was restored and the
mobilizable vector pBHR1 was created. All other features of this vector are similar to pBBR122 described
above.
Host bacteria suited for pBBR122 and pBHR1:
· Aeromonas caviae
· Aeromonas hydrophila
· Acetobacter xylinum
· Aeromonas veronii biovar sobria
· Agrobacterium tumefaciens
· Alcaligenes eutrophus
· Azorizobium caulinodans
· Bartonella bacilifonis
· Bordetella spp
· Brucela spp
· Caulobacter crescentus
· Escherichia coli
· Hyphomicrobium denitrificans
· Hyphomicrobium facilis
· Methylobacilus glyocogenes
· Methylbacterium extorquens
· Methylophilus methylotrophus
Quality Warranty
DNA concentration and purity was checked by UV spectrophotometry. All restriction sites specified in the
vector map were checked by sequencing.
References
Antonie, R. & Locht, C., Mol. Microbiol. 6, 13 (1992) 1785-1799.
Elzer, P.H. et al., Plasmid 33 (1995) 51-57.
Kovach, M.E. et al., Bio Techniques 16, 5 (1994) 800-802.
Kovach, M.E. et al., Gene 166 (1995) 175-176.
Renauld-Mongénie, G. et al., Bacteriol. 178 (1996) 1053-1060.

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