Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]口腔支原体基因组DNA qCRM-23714D BioVector NTCC质粒载体菌种细胞基因保藏中心
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Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]口腔支原体基因组DNA qCRM-23714D
Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]口腔支原体基因组DNA BioVector-qCRM-23714Dwww.biovector.netQuantified genomic DNA isolated from Mycoplasma orale strain CH 19299 [NCTC 10112]. Bacterial genomic DNA is provided in 1X TE Buffer (pH 8.0).Product categoryBacteriaProduct typeNucleic acidCertified reference materialMolecular standardDerived fromMycoplasma orale CH 19299 [NCTC 10112] (ATCC 23714)OrganismMycoplasma oraleType strainYesApplicationsAssay developmentMycoplasma testingNext-generation sequencingQuality controlSpecification range≥ 1 x 106 to 1 x 107 genome copies/μLProduct formatFrozenStorage conditions-70°C or colderGeneralSpecific applicationsQuantitative external control produced under an ISO 17034 accredited process for use in testing and calibration in ISO 17025 accredited laboratories, inclusivity/exclusivity testing, establishing limits of detection, verification or comparison of test methods, and other molecular applications.CharacteristicsCommentsGenome copy number is based on the quantification of the 16S rRNA gene from nine averaged samples. Mycoplasma orale (ATCC 23714) has one copy of the 16S rRNA gene per cell.The reported genome copy number may vary depending on quantitation method used.The limits of detection for this nucleic acid preparation will depend on the set of primers used. Primer efficiency should be determined empirically.This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.Handling informationHandling procedureThaw the vial at room temperature.Avoid exposing genomic DNA to repeated freeze-thaw cycles. Subjecting genomic DNA to repeated free/thaw cycles may result in degradation of the DNA and variations in copies/µL.Handling notesThis preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.Quality control specificationsIntegrityIntegrity of DNA was determined by electrophoresis on a 1% agarose gel stained with ethidium bromide.Functional testsFunctional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.IdentityIdentity confirmed by sequencing of 16S ribosomal RNA gene.Quality accreditationManufactured under an ISO 17034 accredited process
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]口腔支原体基因组DNA BioVector-qCRM-23714Dwww.biovector.netQuantified genomic DNA isolated from Mycoplasma orale strain CH 19299 [NCTC 10112]. Bacterial genomic DNA is provided in 1X TE Buffer (pH 8.0).Product categoryBacteriaProduct typeNucleic acidCertified reference materialMolecular standardDerived fromMycoplasma orale CH 19299 [NCTC 10112] (ATCC 23714)OrganismMycoplasma oraleType strainYesApplicationsAssay developmentMycoplasma testingNext-generation sequencingQuality controlSpecification range≥ 1 x 106 to 1 x 107 genome copies/μLProduct formatFrozenStorage conditions-70°C or colderGeneralSpecific applicationsQuantitative external control produced under an ISO 17034 accredited process for use in testing and calibration in ISO 17025 accredited laboratories, inclusivity/exclusivity testing, establishing limits of detection, verification or comparison of test methods, and other molecular applications.CharacteristicsCommentsGenome copy number is based on the quantification of the 16S rRNA gene from nine averaged samples. Mycoplasma orale (ATCC 23714) has one copy of the 16S rRNA gene per cell.The reported genome copy number may vary depending on quantitation method used.The limits of detection for this nucleic acid preparation will depend on the set of primers used. Primer efficiency should be determined empirically.This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.Handling informationHandling procedureThaw the vial at room temperature.Avoid exposing genomic DNA to repeated freeze-thaw cycles. Subjecting genomic DNA to repeated free/thaw cycles may result in degradation of the DNA and variations in copies/µL.Handling notesThis preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.Quality control specificationsIntegrityIntegrity of DNA was determined by electrophoresis on a 1% agarose gel stained with ethidium bromide.Functional testsFunctional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.IdentityIdentity confirmed by sequencing of 16S ribosomal RNA gene.Quality accreditationManufactured under an ISO 17034 accredited process
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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