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pFUSE-CHIg-hG1e2 Heavy Chain Constant Region Expression plasmid - Engineered Human IgG1质粒载体-BioVector质粒保藏中心

  • 价  格:¥49865
  • 货  号:pFUSE-CHIg-hG1e2 Heavy Chain Constant Region Expression plasmid - Engineered Human IgG1
  • 产  地:北京
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Name质粒名称:pFUSE-CHIg-hG1e2 Heavy Chain Constant Region Expression plasmid - Engineered Human IgG1
Host宿主:
Description描述:Engineered pFUSE-CHIg Engineered Heavy chain (CH) Engineered pFUSE-CHIg express mutant forms of the constant regions of the heavy chain (CH) of the human IgG1. They contain a multiple cloning site (MCS) upstream of these constant regions to enable the cloning of the variable (VH) regions of a given antibody. Engineered Fc alow the production of IgG with modified effector function. More info on Engineered Fc Regions All engineered pFUSE-CHIg plasmids are also available with the IL2 signal sequence to allow the secretion of recombinant IgGs generated using Fab or scFv fragments selected from phage display libraries that lack a signal sequence. pFUSE-CLIg and pFUSE-CHIg plasmids are designed to change a monoclonal antibody from one isotype to another or to produce entire IgG antibodies from Fab or scFv fragments. Transfection of mammalian cell lines with the recombinant pFUSE-CHIg and pFUSE-CLIg pair allows to generate an IgG antibody that can be purified from the supernatant using the appropriate Protein A, Protein G or Protein L affinity chromatography.
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