Streptomyces milbemycinicus CGMCC 7677 strain米尔贝霉素高产链霉菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Streptomyces milbemycinicus CGMCC 7677
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Streptomyces milbemycinicus CGMCC 7677 strain米尔贝霉素高产链霉菌菌株
A Streptomyces hygroscopicus HS7523, which is deposited with an accession number of CGMCC No. 9672 and has the ability to produce milbemycin.The use of Streptomyces HS7523 according to claim 1 for the production of milbemycin.A method for fermentation of milbemycin, comprising the step of aerobic fermentation of Streptomyces HS7523 in a nutrient medium containing assimilable carbon source and assimilable nitrogen source.The method according to claim 3, wherein the assimilable carbon source is selected from one of starch, dextrin, glucose, industrial molasses, glycerol, sucrose, lactose, maltose, trehalose, xylan, mannitol and sorbitol or a combination of the above substances.The method according to claim 3, wherein the assimilable nitrogen source is selected from one of yeast extract, yeast powder, beef extract, tryptone, peptone, skim milk powder, whole milk powder, soybean cake powder, cottonseed cake powder, peanut cake powder, gluten powder, corn pulp dry powder, bran, urea and ammonium salt or a combination of the above substances.The method according to any one of claims 3-5, wherein the adopted milbemycin-producing strain is Streptomyces HS7523 (CGMCC No. 9672), a spontaneous mutant or a mutant obtained by conventional mutagenesis of Streptomyces HS7523 (CGMCC No. 9672).The method according to any one of claims 3-5, wherein the nutrient medium contains 2-10 g/L yeast extract, yeast extract paste or peptone, 20-200 g/L sucrose or molasses, 2-11 g/L skim milk powder or corn pulp, 2-11g/L soybean cake powder, 5-15 g/L cottonseed cake powder or gluten powder, 0.5-1 g/L K2HPO4, 0.05-0.1 g/L FeSO4•7H2O, 0.005-0.02 g/L ZnSO4, 1-5 g/L CaCO3, 0.01-0.05 g/L CuSO4 and /or 0.1-0.5 g/L Na2MoO4.The method according to claim 3, wherein the temperature of the fermentation is 20-40 °C, preferably 25-35°C; pH of the medium is 6.0-8.0, preferably 7.0; the fermentation time is 300-360 h.The method according to any one of claims 3-5, wherein the dissolved oxygen is not less than 35% during the fermentation.The method according to any one of claims 3-5, wherein the ventilation volume is 0.5-1.0vvm during the fermentation.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
A Streptomyces hygroscopicus HS7523, which is deposited with an accession number of CGMCC No. 9672 and has the ability to produce milbemycin.The use of Streptomyces HS7523 according to claim 1 for the production of milbemycin.A method for fermentation of milbemycin, comprising the step of aerobic fermentation of Streptomyces HS7523 in a nutrient medium containing assimilable carbon source and assimilable nitrogen source.The method according to claim 3, wherein the assimilable carbon source is selected from one of starch, dextrin, glucose, industrial molasses, glycerol, sucrose, lactose, maltose, trehalose, xylan, mannitol and sorbitol or a combination of the above substances.The method according to claim 3, wherein the assimilable nitrogen source is selected from one of yeast extract, yeast powder, beef extract, tryptone, peptone, skim milk powder, whole milk powder, soybean cake powder, cottonseed cake powder, peanut cake powder, gluten powder, corn pulp dry powder, bran, urea and ammonium salt or a combination of the above substances.The method according to any one of claims 3-5, wherein the adopted milbemycin-producing strain is Streptomyces HS7523 (CGMCC No. 9672), a spontaneous mutant or a mutant obtained by conventional mutagenesis of Streptomyces HS7523 (CGMCC No. 9672).The method according to any one of claims 3-5, wherein the nutrient medium contains 2-10 g/L yeast extract, yeast extract paste or peptone, 20-200 g/L sucrose or molasses, 2-11 g/L skim milk powder or corn pulp, 2-11g/L soybean cake powder, 5-15 g/L cottonseed cake powder or gluten powder, 0.5-1 g/L K2HPO4, 0.05-0.1 g/L FeSO4•7H2O, 0.005-0.02 g/L ZnSO4, 1-5 g/L CaCO3, 0.01-0.05 g/L CuSO4 and /or 0.1-0.5 g/L Na2MoO4.The method according to claim 3, wherein the temperature of the fermentation is 20-40 °C, preferably 25-35°C; pH of the medium is 6.0-8.0, preferably 7.0; the fermentation time is 300-360 h.The method according to any one of claims 3-5, wherein the dissolved oxygen is not less than 35% during the fermentation.The method according to any one of claims 3-5, wherein the ventilation volume is 0.5-1.0vvm during the fermentation.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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