Fuw-dCas9-Tet1CD-P2A-BFP plasmid vector哺乳动物慢病毒基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Fuw-dCas9-Tet1CD-P2A-BFP plasmid vector哺乳动物慢病毒基因编辑质粒载体

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

BACKBONE
Vector backboneFuw
(Search Vector Database)
Backbone size w/o insert (bp)9216
Total vector size (bp)16359
Modifications to backboneFuw-AscI-EcoRI-HA-P2A-tagBFP
Vector typeMammalian Expression, Lentiviral
Selectable markersZeo marker is outside the LTRs and will not be packaged into virus
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature30°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namedCas9-Tet1CD-HA-P2A-BFP
SpeciesSynthetic
Insert Size (bp)7143
MutationdCas9-D10A, dCas9-H840A
PromoterUbc
Tag / Fusion Protein
HA
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteAscI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primeraactatgcgctcggg
3′ sequencing primertaaagcagcgtBioVector

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net