NCI-H1781 [H1781] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：NCI-H1781 [H1781]
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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NCI-H1781 [H1781] cell line细胞株
NCI-H1781 [H1781]CRL-5894™Product categoryHuman cellsOrganismHomo sapiens, humanTissueLungDiseaseAdenocarcinoma; Bronchoalveolar carcinoma; Stage 3BApplications3D cell cultureProduct formatFrozenCharacteristicsGrowth propertiesAdherentDerivationThe line was established in November 1987.Age66 yearsEthnicityWhiteGenderFemaleClinical dataThe patient received prior chemotherapy and radiation therapy. The patient was a smoker.MetastaticPleural effusionHandling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureRemove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO Quality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: XCSF1PO: 10D13S317: 14D16S539: 8D5S818: 10D7S820: 9,10THO1: 9TPOX: 10,11vWA: 14
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
NCI-H1781 [H1781]CRL-5894™Product categoryHuman cellsOrganismHomo sapiens, humanTissueLungDiseaseAdenocarcinoma; Bronchoalveolar carcinoma; Stage 3BApplications3D cell cultureProduct formatFrozenCharacteristicsGrowth propertiesAdherentDerivationThe line was established in November 1987.Age66 yearsEthnicityWhiteGenderFemaleClinical dataThe patient received prior chemotherapy and radiation therapy. The patient was a smoker.MetastaticPleural effusionHandling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureRemove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO Quality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: XCSF1PO: 10D13S317: 14D16S539: 8D5S818: 10D7S820: 9,10THO1: 9TPOX: 10,11vWA: 14
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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