- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
SNK16 cell line细胞株
SNU-16Cat# BioVector-924175SNU-16 is a cell line exhibiting epithelial morphology that was isolated in 1987 from ascites derived from a 33-year-old, female, Asian, stomach cancer patient prior to chemotherapy. Use these cells in cancer research. Product categoryHuman cellsOrganismHomo sapiens, humanMorphologyepithelialTissueStomachDiseaseGastric CarcinomaApplications3D cell cultureCancer researchProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenCharacteristicsGrowth propertiesSuspension, multicellular aggregatesDerivationSNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. Age33 yearsEthnicityAsianGenderFemaleClinical dataThe line was established from cells taken prior to chemotherapy.KaryotypeThis is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.TumorigenicYes, the cells have a reported colony forming efficiency of 10% in semisolid medium.; myc+; erb-B2+MetastaticAscitesAntigen expressionBlood Type A; Rh +The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.Genes expressedmyc+; erb B2+; Rh+; carcinoembryonic antigen (CEA); TAG 72; blood type AExpression markersVasoactive intestinal peptide (VIP), expressedCommentsSNU-16 cells were positive for VIP receptors but lacked gastrin receptors.The line was established from cells taken prior to chemotherapy.No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditionsTransfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureCultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (BioVector 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 27 hrsSTR profilingAmelogenin: XCSF1PO: 12D13S317: 8,12D16S539: 11,13D5S818: 10,13D7S820: 12THO1: 6,9TPOX: 11vWA: 16
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
SNU-16Cat# BioVector-924175SNU-16 is a cell line exhibiting epithelial morphology that was isolated in 1987 from ascites derived from a 33-year-old, female, Asian, stomach cancer patient prior to chemotherapy. Use these cells in cancer research. Product categoryHuman cellsOrganismHomo sapiens, humanMorphologyepithelialTissueStomachDiseaseGastric CarcinomaApplications3D cell cultureCancer researchProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenCharacteristicsGrowth propertiesSuspension, multicellular aggregatesDerivationSNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. Age33 yearsEthnicityAsianGenderFemaleClinical dataThe line was established from cells taken prior to chemotherapy.KaryotypeThis is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.TumorigenicYes, the cells have a reported colony forming efficiency of 10% in semisolid medium.; myc+; erb-B2+MetastaticAscitesAntigen expressionBlood Type A; Rh +The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.Genes expressedmyc+; erb B2+; Rh+; carcinoembryonic antigen (CEA); TAG 72; blood type AExpression markersVasoactive intestinal peptide (VIP), expressedCommentsSNU-16 cells were positive for VIP receptors but lacked gastrin receptors.The line was established from cells taken prior to chemotherapy.No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditionsTransfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureCultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (BioVector 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 27 hrsSTR profilingAmelogenin: XCSF1PO: 12D13S317: 8,12D16S539: 11,13D5S818: 10,13D7S820: 12THO1: 6,9TPOX: 11vWA: 16
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 SNK16 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
