rh7777-luc cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

rh7777-luc cell line细胞株

I. Introduction
Host Cell: Mca-RH7777
Expressed gene: Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Rattus norvegicus, rat
Tissue: Lliver
Disease: Hepatoma; Morris hepatoma 7777
Morphology: Epitheloid cell
Growth Properties: Loosely adherent

III. Representative Data

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 520±22(Mean±SD) on Mca-RH7777 cells while 528761506±6880441(Mean±SD) on Mca-RH7777-Luc cells.


IV. Stablity Test

Figure 2. Stablity test results. Mca-RH7777-Luc cells showed at least 32 passage stability with similar luminescence unit.

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