小鼠肝内胆管细胞癌细胞系intrahepatic cholangiocarcinoma cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

小鼠肝内胆管细胞癌细胞系intrahepatic cholangiocarcinoma cell line

Intrahepatic cholangiocarcinoma (ICC) is a fatal yet understudied primary liver malignancy. To accelerate the functional annotation of cancer genes and therapeutic targets in this disease, we generate a highly flexible orthotopic allograft mouse model of ICC that can be easily modified in vitro to mimic either oncogene expression by retrovirus-mediated gene transfer or tumor-suppressor gene loss by using RNA interference technology. We use this model to demonstrate that the fused-in-glioblastoma-c-ros-oncogene 1 (FIG-ROS) fusion, which is found in a subset of ICC patients, accelerates cholangiocarcinogenesis. Moreover, by using reversible gene expression, we show that FIG–ROS inactivation in carcinomas harboring mutant kirsten rat sarcoma viral oncogene homolog (Kras) and p53 mutations can potently inhibit tumor growth, thereby validating ROS as a therapeutic target in ICC.

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