HEK293 TLR9/NFkB-Luc cell line基因稳转荧光表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HEK293 TLR9/NFkB-Luc cell line基因稳转荧光表达细胞株

I. Background
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human TLR9 (Toll-like receptor 9, also been designated as CD289, a member of the toll-like receptor (TLR) family. GenBank Accession No. NM_017442).


II. Introduction
Host Cell: HEK293
Expressed gene: TLR9、NFkB-Luc
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): TLR, TLR-9, tlr, nfkb, nf-kb
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:
MEM+10%FBS+1% non-essential amino acids(NEAA)+1 mM Na pyruvate+50 μg/ml Hygromycin B+2ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay

III. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent

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