DH10BacLoxPCre E.coli strain大肠杆菌宿主菌杆状病毒系统菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥59865
- 货 号：DH10BacLoxPCre
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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DH10BacLoxPCre E.coli strain大肠杆菌宿主菌杆状病毒系统菌株
The MultiBac system in a schematic view. Genes of interest are assembled into multigene expression cassettes using the multiplication module present on transfer vectors pFBDM and pUCDM. The resulting vectors are introduced into MultiBac baculoviral DNA in DH10MultiBacCre E. coli cells which contain the factors for Tn7 transposition (for pFBDM derivatives) and cre-lox site-specific recombination (for pUCDM derivatives). Colonies containing bacmid carrying integrated multigene cassettes are identified by blue/white screening (Tn7 transposition disrupts a lacZα gene) and chloramphenicol resistance (conferred by Cre catalyzed integration of pUCDM derivative). Transposition and site-specific recombination are carried out either sequentially or, alternatively, concomitantly in a one-step reaction. Bacmid DNA is prepared from selected clones and used to transfect insect cells for protein production.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
The MultiBac system in a schematic view. Genes of interest are assembled into multigene expression cassettes using the multiplication module present on transfer vectors pFBDM and pUCDM. The resulting vectors are introduced into MultiBac baculoviral DNA in DH10MultiBacCre E. coli cells which contain the factors for Tn7 transposition (for pFBDM derivatives) and cre-lox site-specific recombination (for pUCDM derivatives). Colonies containing bacmid carrying integrated multigene cassettes are identified by blue/white screening (Tn7 transposition disrupts a lacZα gene) and chloramphenicol resistance (conferred by Cre catalyzed integration of pUCDM derivative). Transposition and site-specific recombination are carried out either sequentially or, alternatively, concomitantly in a one-step reaction. Bacmid DNA is prepared from selected clones and used to transfect insect cells for protein production.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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