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Jurkat-RFP cell line人急性T淋巴细胞白血病红色荧光稳转表达永生化细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98965
  • 货  号:jurkat-rfp
  • 产  地:北京
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Jurkat-RFP cell line人急性T淋巴细胞白血病红色荧光稳转表达永生化细胞株

Description
Jurkat cells stably expressing RFP

Species Human (H. sapiens)
Tissue/Organ/Organ System Blood
Growth Properties Suspension
Cell Morphology Round
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Reporter RFP
Unit quantity 1x106 cells / 1.0 ml

Propagation Requirements
The base medium for this cell line is Prigrow II medium available at NTCC Cat. No. NTCC-TM002. To make the completed growth medium, add the following components to the base medium with the final concentration: fetal bovine serum (NTCC-TM999) to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 1.2μg/ml Puromycin. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Expression Profile
Puromycin

QC
1) Puromycin drug selection.2) RFP fluorescence expression.

Disclaimer

1. For for-profit organizations and corporations, please contact BioVector@163.com for pricing of this item.


2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at BioVector@163.com.


3. All test parameters provided in the CoA are conducted using NTCC's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# NTCC-C138). All sales are final.


4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).


5. All of NTCC's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. NTCC is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.


6. NTCC makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. NTCC does not warrant that such information has been shown to be accurate.

7. NTCC warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable NTCC Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Supplier来源:BioVector NTCC Inc.
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