pKNOCK-bla-ermGa拟杆菌自杀质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pKNOCK-bla-ermGa拟杆菌自杀质粒载体

pKNOCK-bla-ermGa/b suicide vectors (Structure 16: 1-11). Base suicide vectors contain ermG for selection on erythromycin in
Bacteroides spp. and bla for selection on ampicillin in E. coli. Vectors are derivatives of pKNOCK-cm (Biotechniques 26(5): 824-828).
The complete nucleotide sequence has been deduced. The pBR325 cat gene was removed from pKNOCK-cm by MluI digestion and replaced
with a PCR-amplified, bla-ermG-containing fragment from pGERM (Methods 20: 35-46). The added bla-ermG fragment, with MluI ends,
was inserted into the pKNOCK-cm MluI sites in both possible orientations (“a” and “b” designation). Select restriction sites are shown along
with the multiple cloning site (MCS), R6K replication origin (oriR6K) and the RP4 transfer origin (RP4 oriT). In general, the “b” version was
used for downstream constructions.

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