Sporomusa ovata strain菌株 35899 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：Sporomusa ovata
- 产 地：北京
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Sporomusa ovata strain菌株 35899
Sporomusa ovata Moller et al.BioVector35899™Type strainProduct categoryBacteriaProduct typeAnaerobeStrain designationDSM 2662Type strainYesIsolation sourceSugar beet leaf silageGeographical isolationGermany; West GermanyProduct formatFreeze-driedStorage conditions2°C to 8°CHandling informationMediumBioVector's Medium 1425: Sporomusa mediumTemperature30°CAtmosphereAnaerobicHandling procedureOpen vial according to enclosed instructions.Under anaerobic conditions, withdraw 0.5 mL of recommended broth from a single test tube (5 to 6 mL) and rehydrate the vial contents.Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 mL each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.Incubate tubes and plate under anaerobic conditions at 30°C. Incubate blood plate aerobically at 37°C.Within 3 to 5 days, growth should be evident by turbidity in the broth. On blood agar plates, colonies are pinpoint, translucent, entire, smooth, and glistening. Growth is best in broth. ANAEROBIC CONDITIONS: Anaerobic conditions for transfer may be obtained by either of the following:Use of an anaerobic gas chamber, or Placement of test tubes under a gassing cannula system hooked to anaerobic gas. Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in anaerobic chamber, Loose screw caps on test tubes in an activated anaerobic gas pack jar, or Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained. Handling notesAnaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology and purity.Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Sporomusa ovata Moller et al.BioVector35899™Type strainProduct categoryBacteriaProduct typeAnaerobeStrain designationDSM 2662Type strainYesIsolation sourceSugar beet leaf silageGeographical isolationGermany; West GermanyProduct formatFreeze-driedStorage conditions2°C to 8°CHandling informationMediumBioVector's Medium 1425: Sporomusa mediumTemperature30°CAtmosphereAnaerobicHandling procedureOpen vial according to enclosed instructions.Under anaerobic conditions, withdraw 0.5 mL of recommended broth from a single test tube (5 to 6 mL) and rehydrate the vial contents.Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 mL each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.Incubate tubes and plate under anaerobic conditions at 30°C. Incubate blood plate aerobically at 37°C.Within 3 to 5 days, growth should be evident by turbidity in the broth. On blood agar plates, colonies are pinpoint, translucent, entire, smooth, and glistening. Growth is best in broth. ANAEROBIC CONDITIONS: Anaerobic conditions for transfer may be obtained by either of the following:Use of an anaerobic gas chamber, or Placement of test tubes under a gassing cannula system hooked to anaerobic gas. Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in anaerobic chamber, Loose screw caps on test tubes in an activated anaerobic gas pack jar, or Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained. Handling notesAnaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology and purity.Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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