pRI101-ON单子叶植物表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRI101-ON单子叶植物表达载体质粒

pRI 101 DNA series are binary vectors for expressing foreign gene in plant cells, which has
the 35S promoter of cauliflower mosaic virus (CaMV) and a 5’non-coding region (5’-UTR) of
alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translational enhancer
in plants1). There are two types of vectors, pRI 101-AN DNA having a 5’-UTR of Arabidopsis
ADH (AtADH 5’-UTR) and pRI 101 ON DNA having a 5’-UTR of rice ADH (OsADH 5’-UTR). The
pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is
for monocotyledonous plants such as rice.
The pRI 101 DNA are shuttle vectors, and replicates autonomously in E. coli and Rhizobium
(Agrobacterium). In E. coli, these vectors are high copy number plasmid because these have
replication origin same as that of pUC type plasmid (ColE1 ori), and these are maintained
stably in Rhizobium (Agrobacterium) also with mutant type replication origin of Ri plasmid
(Ri-ori)2). The pRI 101 DNA are possible to integrate target gene in plant chromosome stably
because the cloning site of these products are located at the position closer to Right Border
(RB) of T-DNA than the selection marker for plant, so the target gene is not deleted.

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