pJET-pyr4 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：pJET-pyr4
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pJET-pyr4 plasmid vector质粒载体
For the construction of pJET-pyr4, the pyr4 gene, including its own promoter and terminator, was amplified by PCR with the primers pyr4_Prom_ClaI and pyr4_Term_EcoRI using chromosomal DNA of T. reesei QM6a as a template and inserted into EcoRV-digested pJET1.2 in the same orientation as the eco47IR gene.For the construction of pCD-Δypr1, the 5′- and 3′-flanks of ypr1 were amplified by PCR with the primers 102499_5fwd_NotI and 102499_5rev_BglII or the primers 102499_3fwd_XbaI and 102499_3rev_HindIII, respectively, using chromosomal DNA of T. reesei QM6a as the template. The 3′-flank was inserted into pJET-pyr4 via restriction with XbaI and HindIII, and then the 5′-flank was added using NotI and BglII.For the construction of pJET-ReYpr1, the ypr1 gene, including its own promoter and terminator, was amplified by PCR with the primers 102499_5fwd3 and 102499_3rev2 using chromosomal DNA of T. reesei QM6a as the template and inserted into EcoRV-digested pJET1.2.For the construction of pCD-Δypr2, the 5′- and 3′-flanks of ypr2 were amplified by PCR with the primers 102497_5fwd_NotI and 102497_5rev_Kpn2I or the primers 102497_3fwd_XbaI and 102497_3rev_HindIII, respectively, using the chromosomal DNA of T. reesei QM6a as the template. The 3′-flank was inserted into pJET-pyr4 via restriction with XbaI and HindIII, and then the 5′-flank was added using NotI and Kpn2I.For the construction of pJET-ReYpr2, the ypr2 gene, including its own promoter and terminator, was amplified by PCR with the primers 102497_5fwd3 and 102497_3rev3 using chromosomal DNA of T. reesei QM6a as the template and inserted into EcoRV-digested pJET1.2.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
For the construction of pJET-pyr4, the pyr4 gene, including its own promoter and terminator, was amplified by PCR with the primers pyr4_Prom_ClaI and pyr4_Term_EcoRI using chromosomal DNA of T. reesei QM6a as a template and inserted into EcoRV-digested pJET1.2 in the same orientation as the eco47IR gene.For the construction of pCD-Δypr1, the 5′- and 3′-flanks of ypr1 were amplified by PCR with the primers 102499_5fwd_NotI and 102499_5rev_BglII or the primers 102499_3fwd_XbaI and 102499_3rev_HindIII, respectively, using chromosomal DNA of T. reesei QM6a as the template. The 3′-flank was inserted into pJET-pyr4 via restriction with XbaI and HindIII, and then the 5′-flank was added using NotI and BglII.For the construction of pJET-ReYpr1, the ypr1 gene, including its own promoter and terminator, was amplified by PCR with the primers 102499_5fwd3 and 102499_3rev2 using chromosomal DNA of T. reesei QM6a as the template and inserted into EcoRV-digested pJET1.2.For the construction of pCD-Δypr2, the 5′- and 3′-flanks of ypr2 were amplified by PCR with the primers 102497_5fwd_NotI and 102497_5rev_Kpn2I or the primers 102497_3fwd_XbaI and 102497_3rev_HindIII, respectively, using the chromosomal DNA of T. reesei QM6a as the template. The 3′-flank was inserted into pJET-pyr4 via restriction with XbaI and HindIII, and then the 5′-flank was added using NotI and Kpn2I.For the construction of pJET-ReYpr2, the ypr2 gene, including its own promoter and terminator, was amplified by PCR with the primers 102497_5fwd3 and 102497_3rev3 using chromosomal DNA of T. reesei QM6a as the template and inserted into EcoRV-digested pJET1.2.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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