pCP-tRNA plasmid vector热带念珠菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：pCP-tRNA
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pCP-tRNA plasmid vector热带念珠菌基因编辑质粒载体
CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisThe plasmid contains the following relevant inserts: 1. C. parapsilosis Autonomously Replicating Sequence 7 for episomal propagation in C. parapsilosis; 2. CAS9 sequence expressed from a C. parapsilosis promoter; 3. Cassette for the expression of the sgRNA from a C. parapsilosis RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisThe plasmid contains the following relevant inserts: 1. C. parapsilosis Autonomously Replicating Sequence 7 for episomal propagation in C. parapsilosis; 2. CAS9 sequence expressed from a C. parapsilosis promoter; 3. Cassette for the expression of the sgRNA from a C. parapsilosis RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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