Reporter gene minicircles报告基因微环载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Reporter gene minicircles报告基因微环载体

Reporter Gene Minicircle报告基因微环载体
BioVector NTCC Inc. offers reporter gene minicircles (luc, lacZ, GFP) as BioVector's McBox.

Compare minicircle DNA with the corresponding plasmid in your experimental setting!
Optimize your transfection!
BioVector NTCC Inc. has brought the first minicircle DNA product to market:

The BioVector's McBox contains 100 µg each of certified minicircle and plasmid DNA which both include the reporter gene.
Currently the McBox is availabe with the luciferase gene (luc) as well as the green fluorescent protein (GFP) and the ß-galactosidase gene (lacZ).

With this new McBox scientists get the opportunity to directly compare minicircle DNA and the corresponding “classical” plasmid in the individual applications in their own labs.

Plasmids and minicircles to go –
immediate delivery off the shelf and ready to use
Reporter gene plasmids with luc, GFP, lacZ

Our service includes
• reproducible quality of the DNA by our standardized manufacturing
technology
• incl. verifi ed LPS-endotoxin removal
• fermentation without any animal derived component
• exactly defi ned quality and a homogeneous product supported by our
proprietary capillary gel electrophoresis (CGE) analysis technology for the
quantifi cation of different plasmid and minicircle topologies
• complete characterization of the product by LPS assay, BCA assay,
UV spectroscopy, restriction analysis, detection of RNA and bacterial
chromosomal DNA by agarose gel electrophoresis (AGE)

Reporter gene and control plasmid features
Available plasmid

pUC21* – PF450 3266 bp Control plasmid, Amp resistance
pUK21* – PF451 3137 bp Control plasmid, Kan resistance
pCMV CMV/– PF464 3546 bp Control plasmid, Amp resistance
pCMV-luc CMV/fi refl y–luciferase PF461 6233 bp Amp resistance
pCMV-lacZ CMV/β–galactosidase PF462 7164 bp Amp resistance
pCMV-GFP CMV/green fluorescent protein PF463 3487 bp Kan resistance


BioVector's McBox luc (Item no. PFBox101)
MC.CMV-luc (minicircle, 100µg)
pCMV-luc (plasmid, 100µg)
WFI (water for injection, 500µL)

BioVector's McBox®lacZ (Item no. PFBox103)
MC.CMV-lacZ (minicircle, 100µg)
pCMV-lacZ (plasmid, 100µg)
WFI (water for injection, 500µL)


BioVector's McBox®GFP (Item no. PFBox102)
MC.CMV-GFP (minicircle, 100µg)
pCMV-GFP (plasmid, 100µg)
WFI (water for injection, 500µL)


Further In Stock products
AAV Helper & Packaging plasmids
• pEPito and pEPI plasmids containing S/MAR elements




Customized Minicircle
According to prior agreement we produce your individual minicircle, using our unique, patented technology.

Your plasmid containing the “Gene of Interest” (GOI) serves as starting material. Either by cut and paste or amplification by PCR, this will be inserted in to the so called parental plasmid. Intramolecular recombination of this parental plasmid leads to the minicircle DNA molecule containing almost only the GOI.
The minicircle DNA based on our proprietary minicircle technology is worldwide patented.

Minicircle - minimized DNA
for maximized success
Minicircle features
• no bacterial backbone sequences
(e.g. ori, antibiotic resistance marker)
• supercoiled monomer of minimal size
• highly purifi ed
• reduced CpG content
• improved safety
• minimized side effects
• excellent transfection effi ciency

Production procedure
Your plasmid containing the gene of interest (GOI) serves as starting material.
Either by cut and paste, gene synthesis or amplification by PCR, this will be inserted
into the so-called parental plasmid. Intramolecular recombination of this parental
plasmid leads to the minicircle DNA molecule containing almost only the GOI

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net