Hep-53.4 cell line小鼠肝细胞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Hep-53.4 cell line小鼠肝细胞癌细胞株

Hep-53.4 Cell Line
Cat No.: NTCC921779

Cell line name Hep-53.4
Synonyms HEP-53.4; 53.4

Comments Transformant: ChEBI; CHEBI:34873; N-nitrosodiethylamine (Diethylnitrosamine; DEN).
Derived from sampling site: Liver.
Disease Hepatocellular carcinoma of the mouse (NCIt: C38756)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6J.

Sex of cell Female
Age at sampling Adult
Category Cancer cell line
Morphology
Epithelial-like
Growth properties
Adherent
Description
Established from the primary hepatocellular carcinoma of C57BL/6J mice.

Special Features of the Cell Line
Tumorigenic
Yes, in C57BL/6J mice
Viruses
SMRV: Negative, as confirmed by Real
-
Time PCR
Mutational
profile
p53 wt
Species
The mouse origin was verified by Real
-
Time
-
PCR.
Breed/Subspecies
C57BL/6J

HLA-typing:


Proliferating cultures
The cell culture flasks, 2xT25, come filled with cell culture medium. Collect the entire medium in 2 x 50 ml centrifuge tubes. Carefully add 5 ml of cell culture medium to each of the two T25 cell culture flasks. Control the cell morphology and confluency under the microscope. Incubate at 37 °C for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to 1 x T25 cell culture. Incubate at 37 °C for a minimum of 24 hours.

Sterility Plasmotest: negative; Mycoplasma specific PCR: negative

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