pTnRSVneo-loxP, pTn(minimal)loxP, pTnBAC-loxP转座子质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：pTnRSVneo-loxP, pTn(minimal)loxP, pTnBAC-loxP
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pTnRSVneo-loxP, pTn(minimal)loxP, pTnBAC-loxP转座子质粒
Scheme for selective recovery of PAC and BAC deletions. (A) Insertion of a transposon containing a loxP site, a bacterial selectable marker (BSM) and a mammalian cell selectable marker (MSM) into the genomic insert of a PAC or BAC clone is shown. Cre protein synthesized early during infection by P1 vir s mediates recombination of loxP sites both to create a deletion in the insert and form the P1-PAC/BAC cointegrate. P1 packaging initiates at the pac site (supplied by P1 vir s ) and proceeds till the phage head is full. Packaging of the downstream loxP site and Cre gene enables the DNA between the loxP sites to be recovered as a circular molecule after infection. In the absence of packaging of the downstream loxP site, the DNA is destroyed after infection as indicated in the lower panel. The location and direction of PCR primers used to characterize PAC or BAC deletions are indicated. (B) Structure of four transposon donating plasmids used in this study. Important general features include a transposase gene located outside the 70 bp inverted repeat ends of the transposon (indicated here as open boxes Tn10) and a loxP site and bacterial resistance marker within the transposon boundaries.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Scheme for selective recovery of PAC and BAC deletions. (A) Insertion of a transposon containing a loxP site, a bacterial selectable marker (BSM) and a mammalian cell selectable marker (MSM) into the genomic insert of a PAC or BAC clone is shown. Cre protein synthesized early during infection by P1 vir s mediates recombination of loxP sites both to create a deletion in the insert and form the P1-PAC/BAC cointegrate. P1 packaging initiates at the pac site (supplied by P1 vir s ) and proceeds till the phage head is full. Packaging of the downstream loxP site and Cre gene enables the DNA between the loxP sites to be recovered as a circular molecule after infection. In the absence of packaging of the downstream loxP site, the DNA is destroyed after infection as indicated in the lower panel. The location and direction of PCR primers used to characterize PAC or BAC deletions are indicated. (B) Structure of four transposon donating plasmids used in this study. Important general features include a transposase gene located outside the 70 bp inverted repeat ends of the transposon (indicated here as open boxes Tn10) and a loxP site and bacterial resistance marker within the transposon boundaries.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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