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Yarrowia lipolytica PEX17-HA strain菌株ATCC 201249 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:Yarrowia lipolytica PEX17-HA ATCC 201249
  • 产  地:北京
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Yarrowia lipolytica PEX17-HA strain菌株ATCC 201249

Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx
BioVector-201249™

25 Product Citations
Product category
Fungi
Product type
Yeast
Strain designation
PEX17-HA
Type strain
No
Isolation source
Derived from strain E122

General
Specific applications
able to use oleic acid as carbon source
Preceptrol
No
Characteristics
Ploidy
Haploid
Genotype
MATA ura3-302 leu2-270 lys8-11 PEX17-HA
Comments
regulation of PEX17 gene expression
able to use oleic acid as carbon source
Handling information
Medium
BioVector Medium 1245: YEPD
Temperature
24°C
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.

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