Yarrowia lipolytica PO1f strain解脂耶氏酵母菌株ATCC MYA-2613 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Yarrowia lipolytica PO1f ATCC MYA-2613
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Yarrowia lipolytica PO1f strain解脂耶氏酵母菌株ATCC MYA-2613
Yarrowia lipolytica (Wickerham et al.) van der Walt et von ArxNTCC-MYA-2613Product categoryFungiProduct typeYeastStrain designationPO1fType strainNoGenome sequenced strainYesIsolation sourceDerived from existing strain, W29 (NTCC 20460)GeneralSpecific applicationstransformation hostPreceptrolNoCharacteristicsGenotypeMATA ura3-302 leu2-270 xpr2-322 axp2-deltaNU49 XPR2::SUC2MorphologyOn YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.CommentsUnable to produce extracellular proteaseHandling informationMediumATCC Medium 28: Emmons' modification of Sabouraud's agar/brothATCC Medium 200: YM agar or YM brothATCC Medium 1245: YEPDTemperature24-26°CAtmosphereAerobicHandling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Yarrowia lipolytica (Wickerham et al.) van der Walt et von ArxNTCC-MYA-2613Product categoryFungiProduct typeYeastStrain designationPO1fType strainNoGenome sequenced strainYesIsolation sourceDerived from existing strain, W29 (NTCC 20460)GeneralSpecific applicationstransformation hostPreceptrolNoCharacteristicsGenotypeMATA ura3-302 leu2-270 xpr2-322 axp2-deltaNU49 XPR2::SUC2MorphologyOn YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.CommentsUnable to produce extracellular proteaseHandling informationMediumATCC Medium 28: Emmons' modification of Sabouraud's agar/brothATCC Medium 200: YM agar or YM brothATCC Medium 1245: YEPDTemperature24-26°CAtmosphereAerobicHandling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
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