OVCAR3/DDP cell line人卵巢癌顺铂耐药细胞株OVCAR-3/DDP BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：OVCAR3/DDP
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OVCAR3/DDP cell line人卵巢癌顺铂耐药细胞株OVCAR-3/DDP
A cisplatin-resistant subline, OVCAR3/DDP, was established from the OVCAR3 ovarian cell line. OVCAR3/DDP cells were induced using a progressive concentration of cisplatin. Briefly, OVCAR3 that were sourced from BioVector NTCC Inc. cells in the logarithmic growth phase were treated with 2.5 µmol/l cisplatin. Following 48–72 h, cisplatin was removed and the cells were cultured at 37°C without cisplatin for ~3 weeks until they recovered. Then the cells were treated with 5 µmol/l and 10 µmol/l cisplatin, respectively. The cisplatin resistant OVCAR3/DDP cell line was successfully established when cells survived in 10 µmol/l cisplatin for ~2 months with a normal activity.Organism Homo sapiens, humanTissue ovaryCell Type epithelialProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Disease adenocarcinomaAge 60 yearsGender femaleEthnicity CaucasianApplications This cell line is a suitable transfection host.NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.Karyotype The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.Images Derivation The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.Clinical Data Caucasianfemale60 yearsReceptor Expression Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positiveTumorigenic YesEffects Yes, Forms colonies in soft agarYes, in nude mice inoculated subcutaneously with 10(7) cells(Tumors developed within 21 days at 100% frequency (5/5).)Comments Forms colonies in soft agar and has an abnormal karyotype.Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.Both cultured cells and xenografts exhibit androgen and estrogen receptors.Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.Subculturing Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.1.Remove and discard culture medium.2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting5.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: Every 2 to 3 daysCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CAtmosphere: air, 95%; carbon dioxide (CO2), 5%STR Profile Amelogenin: XCSF1PO: 11,12D13S317: 12D16S539: 12D5S818: 11,12D7S820: 10THO1: 9,9.3TPOX: 8vWA: 17Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 1PGM1, 1PGM3, 1
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
A cisplatin-resistant subline, OVCAR3/DDP, was established from the OVCAR3 ovarian cell line. OVCAR3/DDP cells were induced using a progressive concentration of cisplatin. Briefly, OVCAR3 that were sourced from BioVector NTCC Inc. cells in the logarithmic growth phase were treated with 2.5 µmol/l cisplatin. Following 48–72 h, cisplatin was removed and the cells were cultured at 37°C without cisplatin for ~3 weeks until they recovered. Then the cells were treated with 5 µmol/l and 10 µmol/l cisplatin, respectively. The cisplatin resistant OVCAR3/DDP cell line was successfully established when cells survived in 10 µmol/l cisplatin for ~2 months with a normal activity.Organism Homo sapiens, humanTissue ovaryCell Type epithelialProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Disease adenocarcinomaAge 60 yearsGender femaleEthnicity CaucasianApplications This cell line is a suitable transfection host.NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.Karyotype The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.Images Derivation The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.Clinical Data Caucasianfemale60 yearsReceptor Expression Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positiveTumorigenic YesEffects Yes, Forms colonies in soft agarYes, in nude mice inoculated subcutaneously with 10(7) cells(Tumors developed within 21 days at 100% frequency (5/5).)Comments Forms colonies in soft agar and has an abnormal karyotype.Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.Both cultured cells and xenografts exhibit androgen and estrogen receptors.Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.Subculturing Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.1.Remove and discard culture medium.2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting5.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: Every 2 to 3 daysCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CAtmosphere: air, 95%; carbon dioxide (CO2), 5%STR Profile Amelogenin: XCSF1PO: 11,12D13S317: 12D16S539: 12D5S818: 11,12D7S820: 10THO1: 9,9.3TPOX: 8vWA: 17Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 1PGM1, 1PGM3, 1
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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