pRS-shRNA29基因干扰逆病毒质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRS-shRNA29基因干扰逆病毒质粒载体

Viral Packaging Retroviral
Feature The HuSH pRS plasmid vector incorporates all the elements as above two vectors with the absence of a fluorescence marker. The bacterial selection marker for pRS vector is ampicillin and the mammalian selection can still be achieved with puromycin.

Product Description:
· Plasmid vector for cloning shRNA
expression cassettes
· Designed for long term gene silencing
studies
· Ampicillin (100ug/ml) and Puromycin
resistance markers for easy selection
of transformed or transfected cells
· U6 polymerase III promoter for shRNA
expression
· MMLV LTR sequences for packaging
into retroviral particles
· EcoRI and HindIII sites convenient for
shuttling existing HuSH cassettes
Quality Control Assays
DNA Quantitation: The concentration of the
purified plasmid was determined at OD260 by a
UV spectrometer.
DNA Sequence Analysis: The final purified
plasmid was sequenced to confirm its identity.
Functional Analysis:
1. Cloning: the pRS plasmid was digested with
BamHI and HindIII and the digested fragment
isolated. Multiple shRNA expression
cassettes were cloned into this plasmid.
2. Inhibition of target gene: shRNA constructs
cloned into pRS were verified for inhibition of
target genes.
3. Stable cell lines: pRS was verified to generate
stable cell lines using direct transfection

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net