pFGL821真菌ATMT转化表达载体Hygro潮霉素筛选标记 BioVector NTCC质粒载体菌种细胞基因保藏中心

pFGL821真菌ATMT转化表达载体Hygro潮霉素筛选标记

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

BACKBONE
Vector backbone pFGL815
Modifications to backboneHygromycin B resistance (hptII) cassette (1.4kb) has been cloned into pFGL815 (Addgene 52322) between Xba1 and Sal1 sites.
Vector typeBacterial Expression
Selectable markersHygromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namehygromycin B phosphotransferase
Alt namehptII
SpeciesEscherichia coli
Insert Size (bp)1422
GenBank ID
PromoterAspergillus nidulans trpC promoter
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteXhoI (destroyed during cloning)
3′ cloning siteSalI (not destroyed)
5′ sequencing primerGATCCTCGAGTGATATTGAAGGAGCACTTTTTGGGC
3′ sequencing primerGACGTCGACTGGTTCCGGTCGGCATCTACTCTATT

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net