THP-1 NF-kb luciferase reporter cell line荧光报告细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥498685
- 货 号：THP-1 NF-kb luciferase reporter
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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THP-1 NF-kb luciferase reporter cell line荧光报告细胞株
NF-κB LuciaTM Reporter MonocytesTHP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of secreted luciferase (Lucia).The levels of NF-κB-induced Lucia in the cell culture supernatant are readily assessed with QUANTI-Luc™, a luciferase detection reagent.THP1-Lucia™ cells induce the activation of NF-κB in response to various PRR ligands.THP1-Lucia™ cells are resistant to Zeocin®.SpecificationsAntibiotic resistance: Zeocin®Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)Guaranteed mycoplasma-freeDescriptionTHP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of Lucia luciferase.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
NF-κB LuciaTM Reporter MonocytesTHP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of secreted luciferase (Lucia).The levels of NF-κB-induced Lucia in the cell culture supernatant are readily assessed with QUANTI-Luc™, a luciferase detection reagent.THP1-Lucia™ cells induce the activation of NF-κB in response to various PRR ligands.THP1-Lucia™ cells are resistant to Zeocin®.SpecificationsAntibiotic resistance: Zeocin®Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)Guaranteed mycoplasma-freeDescriptionTHP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of Lucia luciferase.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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