Neelamegham Human GlycoGene CRISPR Library (Pooled Library #140961)基因编辑慢病毒质粒文库 BioVector NTCC质粒载体菌种细胞基因保藏中心

Neelamegham Human GlycoGene CRISPR Library
(Pooled Library #140961)基因编辑慢病毒质粒文库

The GlycoGene CRISPR lentiviral library targets human genes involved in cellular glycosylation. These include glycosyltransferase genes involved in biosynthesis of several different types of glycoconjugates, including: N-glycans, O-glycans, glycolipids, glycosaminoglycans, GPI-anchored carbohydrates, O-fucose, O-GlcNAc, O-Glucose, O-Mannose, and EGF domain specific O-linked GlcNAcTs (EOGTs).

This library also targets proteins involved in intracellular protein transport, sulfotransferases, enzymes involved in nucleotide-sugar biosynthesis, nucleotide-sugar transporters and glycosidases.

VECTOR BACKBONE
SiC-V2-Cas9G7 - does not express Cas9

This vector was modified as described in the associated publication. The mCerulean gene has been replaced with TagBFP. For library construction the sgRNA cloning region of lentiCRISPR V2 was cloned downstream of the U6 promoter.

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