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pTAex3 plasmid vector质粒载体
A. oryzae M-2-3 is an auxotroph for arginine, and pTAex3 has the argB gene from Aspergillus nidulans (10). Although pTAex3 is not an autonomously replicating plasmid in A. oryzae, it is designed for the expression of recombinant proteins by integration into the chromosome of A. oryzae. Therefore, a transformant of A. oryzae M-2-3 having pTAex3 was grown on an arginine-free medium. Furthermore, the plasmid can replicate in Escherichia coli because of the replication origin in pUC119. Construction, propagation, and amplification of the hybrid plasmids were performed with E. coli DH5α or JM109 (Takara Co., Ltd., Tokyo, Japan). E. coli was cultured in Luria-Bertani medium (1% tryptone, 0.5% yeast extract, 1% NaCl [pH 6.8]) according to the standard method
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
A. oryzae M-2-3 is an auxotroph for arginine, and pTAex3 has the argB gene from Aspergillus nidulans (10). Although pTAex3 is not an autonomously replicating plasmid in A. oryzae, it is designed for the expression of recombinant proteins by integration into the chromosome of A. oryzae. Therefore, a transformant of A. oryzae M-2-3 having pTAex3 was grown on an arginine-free medium. Furthermore, the plasmid can replicate in Escherichia coli because of the replication origin in pUC119. Construction, propagation, and amplification of the hybrid plasmids were performed with E. coli DH5α or JM109 (Takara Co., Ltd., Tokyo, Japan). E. coli was cultured in Luria-Bertani medium (1% tryptone, 0.5% yeast extract, 1% NaCl [pH 6.8]) according to the standard method
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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