Steady-Glo uciferase Assay System荧光素酶检测系统试剂盒 BioVector NTCC质粒载体菌种细胞基因保藏中心

Steady-Glo uciferase Assay System荧光素酶检测系统试剂盒

Standard Protocol for the BioVector's Steady Assay System
BioVector's Steady Luciferase Assay System yields reliable and robust results in
high-throughput screening applications. The BioVector's Steady Reagent provides
stable luminescence with a half-life of greater than 5 hours when used with
common cell culture media.
1. Remove 96- or 384-well plates containing mammalian cells from the
incubator. The plates used must be compatible with the luminometer
being used. For best results, equilibrate cultured cells to room
temperature before performing Step 2.
2. To each plate well, add a volume of BioVector's Steady Reagent equal to the
volume of culture medium in the well, and mix. (For 96-well plates,
typically 100µl of reagent is added to cells grown in 100µl of medium.
For 384-well plates, typically 30µl of reagent is added to cells grown
in 30µl of medium.)
3. Wait at least 5 minutes to allow cell lysis, then measure luminescence
in a luminometer.

Characteristics of BioVector's Steady Assay Reagent.

NH = Nonhomogeneous assay format (when used
with Glo Lysis Buffer, Cat.# E2661).
H = Homogeneous assay format.
*Percent change in luminescence was determined as
follows: Reagent was added to all wells, the plate
was read, and the signals from wells 1 and 96 were
compared.


Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net