pJA15-Template for in vitro tRNA expression体外tRNA表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pJA15-Template for in vitro tRNA expression体外tRNA表达载体质粒

This plasmid contains the T7 RNA polymerase promoter and E. coli tRNA His and is used as a template for in vitro transcription of E. coli tRNA His. The tRNA gene of interest can be inserted into the multiple cloning site of the expression plasmid, downstream from the T7 RNA promoter. The tRNA gene terminates with a FokI restriction site, such that run-off transcription of the linearized plasmid produces a tRNA transcript with the correct CCA end.

Product Type: Plasmid
Gene/insert name: T7 RNA polymerase promoter + E.coli tRNA His
Antibiotic Resistance: Ampicillin
Fusion Tag(s): none
Grow in E. coli at 37 C: Yes
Selectable markers: Ampicillin
Cloning Site 5': EcoRI
Cloning Site 3': BamHI
Insert Size: 115 bp
Vector Backbone and Size: pUC19, 2686 bp
High or low copy: High

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