pnCasSA–BEC金黄色葡萄球菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：pnCasSA–BEC
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pnCasSA–BEC金黄色葡萄球菌基因编辑质粒载体
The engineered base-editing system enables highly efficient C → T conversion in the S. aureus RN4220 strain. (A) Map of the base-editing plasmid pnCasSA–BEC. APOBEC1-nCas9, a fusion protein composed of a deaminase APOBEC1 at the N terminus and a Cas9 nickase Cas9D10A at the C terminus; rpsL promoter, an S. aureus strong promoter used to drive the expression of APOBEC1-nCas9; BsaI sites, Golden Gate assembly of spacers; cap 1A promoter, the sgRNA expression promoter; ColE1, an E. coli replication origin. KanR, the antibiotic marker kanamycin used in E. coli; Cm, the chloramphenicol-resistance marker used in S. aureus; repF, an S. aureus temperature-sensitive origin for plasmid curing after editing.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
The engineered base-editing system enables highly efficient C → T conversion in the S. aureus RN4220 strain. (A) Map of the base-editing plasmid pnCasSA–BEC. APOBEC1-nCas9, a fusion protein composed of a deaminase APOBEC1 at the N terminus and a Cas9 nickase Cas9D10A at the C terminus; rpsL promoter, an S. aureus strong promoter used to drive the expression of APOBEC1-nCas9; BsaI sites, Golden Gate assembly of spacers; cap 1A promoter, the sgRNA expression promoter; ColE1, an E. coli replication origin. KanR, the antibiotic marker kanamycin used in E. coli; Cm, the chloramphenicol-resistance marker used in S. aureus; repF, an S. aureus temperature-sensitive origin for plasmid curing after editing.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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