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PACADD-137 cell line细胞株
Cell line: PACADD-137NTCC no.: 931711Species validation: human (Homo sapiens)Cell type: pancreas ductal adenocarcinomaOrigin: established from a primary pancreatic ductal adenocarcinoma of a 75-year-old woman; cells were described in the literature to show nuclear p53 accumulationBiosafety level: 1Cell Culture Data:Morphology: epithelial cells growing adherently in monolayers in tight islands; image; imageMedium: 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBSSubculture: seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 once a week using trypsin/EDTA for 5 minutes; after thawing or trypsinization cells may need 1-2 days to adhereIncubation: at 37 °C with 5% CO2Doubling time: ca. 2-3 daysHarvest: cell harvest of ca. 15 x 106 cells/80 cm2Storage: frozen with 70% medium, 20% FBS, 10% DMSOScientific Data:Mycoplasma: negative in microbiological culture, PCR assaysImmunology: To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Cell line: PACADD-137NTCC no.: 931711Species validation: human (Homo sapiens)Cell type: pancreas ductal adenocarcinomaOrigin: established from a primary pancreatic ductal adenocarcinoma of a 75-year-old woman; cells were described in the literature to show nuclear p53 accumulationBiosafety level: 1Cell Culture Data:Morphology: epithelial cells growing adherently in monolayers in tight islands; image; imageMedium: 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBSSubculture: seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 once a week using trypsin/EDTA for 5 minutes; after thawing or trypsinization cells may need 1-2 days to adhereIncubation: at 37 °C with 5% CO2Doubling time: ca. 2-3 daysHarvest: cell harvest of ca. 15 x 106 cells/80 cm2Storage: frozen with 70% medium, 20% FBS, 10% DMSOScientific Data:Mycoplasma: negative in microbiological culture, PCR assaysImmunology: To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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