- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
PACADD-165 cell line细胞株
Cell line: PACADD-165NTCC No.:931750Species validation: human (Homo sapiens)Cell type: pancreas carcinomaOrigin: established from the ascites of a 54-year-old man with advanced pancreatic ductal adenocarcinoma (stage G3)NTCC Cell Culture Data:Morphology: epithelioid small polygonal cells growing adherently in monolayers; image; imageMedium: 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBSSubculture: seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:3 - 1:10 every 3-6 days using trypsin/EDTA; immediately after recovery the doubling time increases to ca. 70 hoursIncubation: at 37 °C with 5% CO2Doubling time: ca. 35-45 hoursHarvest: cell harvest of ca. 12 x 106 cells/175 cm2Storage: frozen with 70% medium, 20% FBS, 10% DMSONTCC Scientific Data:Mycoplasma: negative in PCR assayImmunology: To inquire about expression of EpCAM and intermediate filaments, contact BioVector NTCC Inc.Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Cell line: PACADD-165NTCC No.:931750Species validation: human (Homo sapiens)Cell type: pancreas carcinomaOrigin: established from the ascites of a 54-year-old man with advanced pancreatic ductal adenocarcinoma (stage G3)NTCC Cell Culture Data:Morphology: epithelioid small polygonal cells growing adherently in monolayers; image; imageMedium: 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBSSubculture: seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:3 - 1:10 every 3-6 days using trypsin/EDTA; immediately after recovery the doubling time increases to ca. 70 hoursIncubation: at 37 °C with 5% CO2Doubling time: ca. 35-45 hoursHarvest: cell harvest of ca. 12 x 106 cells/175 cm2Storage: frozen with 70% medium, 20% FBS, 10% DMSONTCC Scientific Data:Mycoplasma: negative in PCR assayImmunology: To inquire about expression of EpCAM and intermediate filaments, contact BioVector NTCC Inc.Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
- 公告/新闻
