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pPL-PCB原核阿拉伯糖诱导表达质粒载体
Strains harboring the single plasmid, pBAD-Cph1(N514) or pPL-PCB, displayed no visible color change. Crude extracts from cotransformed and single plasmid-containing strains were analyzed for the presence of functional photoconvertible holophytochrome spectrophotometrically. The cotransformed strain exhibited a far-red-minus-red difference spectrum characteristic of photoactive holophytochrome. Extracts from pBAD-Cph1(N514)/pPL-PCB cells exhibited difference maxima and minima at 659 and 704 nm, characteristic of the Cph1-PCB adduct of Cph1 (12). Phytochrome difference spectra were not detectable in extracts from similarly induced cells containing only one of the two plasmid
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Strains harboring the single plasmid, pBAD-Cph1(N514) or pPL-PCB, displayed no visible color change. Crude extracts from cotransformed and single plasmid-containing strains were analyzed for the presence of functional photoconvertible holophytochrome spectrophotometrically. The cotransformed strain exhibited a far-red-minus-red difference spectrum characteristic of photoactive holophytochrome. Extracts from pBAD-Cph1(N514)/pPL-PCB cells exhibited difference maxima and minima at 659 and 704 nm, characteristic of the Cph1-PCB adduct of Cph1 (12). Phytochrome difference spectra were not detectable in extracts from similarly induced cells containing only one of the two plasmid
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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