DG-75 [D.G.-75] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：DG-75 [D.G.-75] cell line
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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DG-75 [D.G.-75] cell line细胞株
DG-75 [D.G.-75]Cat# NTCC-CRL-2625Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphocyteMorphologylymphoblastDiseaseBurkitts LymphomaApplications3D cell cultureImmunologyCharacteristicsGrowth propertiesSuspensionDerivationThe DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitt's lymphoma.Age10 yearsEthnicityWhiteGenderMaleKaryotypet(8:14)(q24;q32)MetastaticPleural effusionAntigen expressionCD10; Homo sapiens, expressedRefCD19; Homo sapiens, expressed (weakly expressed)RefrefCD20; Homo sapiensRefCD23; Homo sapiensRefCD38; Homo sapiens, expressedRefCD39; Homo sapiensRefGenes expressedsurface immunoglobulin (IgM, k)Expression markersFc, not expressed; complement (C3), not expressedCommentsThe cells are reported to be negative for Epstein-Barr virus and E-rosette negative. RefA culture submitted to the NTCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is NTCC-formulated RPMI-1640 Medium, NTCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NTCC 30-2020) to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately two minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend the cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).5. Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product.Subculturing procedureCultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (NTCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 20 to 24 hrs
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
DG-75 [D.G.-75]Cat# NTCC-CRL-2625Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphocyteMorphologylymphoblastDiseaseBurkitts LymphomaApplications3D cell cultureImmunologyCharacteristicsGrowth propertiesSuspensionDerivationThe DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitt's lymphoma.Age10 yearsEthnicityWhiteGenderMaleKaryotypet(8:14)(q24;q32)MetastaticPleural effusionAntigen expressionCD10; Homo sapiens, expressedRefCD19; Homo sapiens, expressed (weakly expressed)RefrefCD20; Homo sapiensRefCD23; Homo sapiensRefCD38; Homo sapiens, expressedRefCD39; Homo sapiensRefGenes expressedsurface immunoglobulin (IgM, k)Expression markersFc, not expressed; complement (C3), not expressedCommentsThe cells are reported to be negative for Epstein-Barr virus and E-rosette negative. RefA culture submitted to the NTCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is NTCC-formulated RPMI-1640 Medium, NTCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NTCC 30-2020) to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately two minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend the cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).5. Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product.Subculturing procedureCultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (NTCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 20 to 24 hrs
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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