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BJ5465 Saccharomyces cerevisiaestrain酿酒酵母菌株单倍体 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49865
  • 货  号:Saccharomyces cerevisiaeBJ5465
  • 产  地:北京
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BJ5465 Saccharomyces cerevisiaestrain酿酒酵母菌株单倍体

BJ5465 Saccharomyces cerevisiae Meyen ex E.C. Hansen
BioVector®208289

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain designation
BJ5465
Type strain
No

Characteristics
Mating type
a
Ploidy
Haploid
Genotype
MATa ura3-52 trp1 leu2-delta1 his3-delta200 pep4::HIS3 prb1-delta1.6R can1 GAL
Comments
Proteinase deficient (CH1)
Handling information
Medium
BioVector® Medium 1069: YPAD medium
BioVector® Medium 1245: YEPD
BioVector® Medium 28: Emmons' modification of Sabouraud's agar/broth
BioVector® Medium 200: YM agar or YM broth
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 10 µL (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1 to 2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

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