Rhodopseudomonas palustris strain沼泽红假单胞菌菌株 17001 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：Rhodopseudomonas palustris (Molisch) van Niel 17001
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Rhodopseudomonas palustris strain沼泽红假单胞菌菌株17001
Rhodopseudomonas palustris (Molisch) van NielNTCC-17001Type Strain. Produces neuraminidase sialidase.Product categoryBacteriaProduct typeExtremophileStrain designationATH 2.1.6 [NTCC 11168, E.II.5.1.6, NCIB 11692, NCIB 8252, NCIB 8288, Pal 6]Type strainYesGenome sequenced strainYesProduct formatFreeze-driedStorage conditions2°C to 8°CGeneralSpecific applicationsProduces neuraminidase sialidaseBiotechnologyPreceptrolNoCharacteristicsExtremophile typePhototrophHandling informationMediumBioVector® Medium 112: Van Niel's yeast agarTemperature30°CAtmosphereAnaerobicIncubationUnder a tungsten lampHandling procedurePut 6 to 8 mL of BioVector® Medium #112 into a small screw cap test tube (13x100 mm). Add 0.1 mL of 3% cysteine per each 5-6 mL of medium and then fill the test tube to capacity with additional Medium #112. Seal the test tube with a screw cap.Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.Open the freeze-dried vial according to enclosed instructions.Aseptically take 0.5 mL of the pre-reduced medium and rehydrate the pellet.Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity). Streak one drop of the rehydrated pellet onto Nutrient Agar (BD 213000) and/or Tryptic Soy Agar (BD 236950).Incubate the broth culture at 30°C under a tungsten lamp. Incubate the aerobic Nutrient Agar plate and/or Tryptic Soy Agar plate at 30°C in the dark.Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (10-20%) the medium does not need to be pre-reduced. Growth should be detected on the Nutrient Agar plate and/or Tryptic Soy Agar plate within 3 to 6 days.When examined microscopically, the cells are motile rods, in single and pairs. When grown in broth, the culture is pink in color.Handling notesColonies on #3 agar appear small, circular, entire, and low convex with a light pink center.This organism should be incubated in a light filled environment.This culture is oxygen-tolerant, therefore strictly anoxic conditions are not required when rehydrating the freeze-dried pellet or transferring the organism.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Rhodopseudomonas palustris (Molisch) van NielNTCC-17001Type Strain. Produces neuraminidase sialidase.Product categoryBacteriaProduct typeExtremophileStrain designationATH 2.1.6 [NTCC 11168, E.II.5.1.6, NCIB 11692, NCIB 8252, NCIB 8288, Pal 6]Type strainYesGenome sequenced strainYesProduct formatFreeze-driedStorage conditions2°C to 8°CGeneralSpecific applicationsProduces neuraminidase sialidaseBiotechnologyPreceptrolNoCharacteristicsExtremophile typePhototrophHandling informationMediumBioVector® Medium 112: Van Niel's yeast agarTemperature30°CAtmosphereAnaerobicIncubationUnder a tungsten lampHandling procedurePut 6 to 8 mL of BioVector® Medium #112 into a small screw cap test tube (13x100 mm). Add 0.1 mL of 3% cysteine per each 5-6 mL of medium and then fill the test tube to capacity with additional Medium #112. Seal the test tube with a screw cap.Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.Open the freeze-dried vial according to enclosed instructions.Aseptically take 0.5 mL of the pre-reduced medium and rehydrate the pellet.Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity). Streak one drop of the rehydrated pellet onto Nutrient Agar (BD 213000) and/or Tryptic Soy Agar (BD 236950).Incubate the broth culture at 30°C under a tungsten lamp. Incubate the aerobic Nutrient Agar plate and/or Tryptic Soy Agar plate at 30°C in the dark.Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (10-20%) the medium does not need to be pre-reduced. Growth should be detected on the Nutrient Agar plate and/or Tryptic Soy Agar plate within 3 to 6 days.When examined microscopically, the cells are motile rods, in single and pairs. When grown in broth, the culture is pink in color.Handling notesColonies on #3 agar appear small, circular, entire, and low convex with a light pink center.This organism should be incubated in a light filled environment.This culture is oxygen-tolerant, therefore strictly anoxic conditions are not required when rehydrating the freeze-dried pellet or transferring the organism.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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