Aspergillus oryzae RIB40 strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Aspergillus oryzae RIB40 strain菌株


NTCC-42149

Product Categories
Fungi, Microorganisms, Fungi

Biosafety Level
1

Product Type
Micro

Strain Designation
RIB40

Organism
Aspergillus oryzae

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Genome sequenced strain
Yes
Isolation source
Cereal
Product format
Freeze-dried
Storage conditions
2°C to 8°C

General
Specific applications
Produces acetamidase
Produces acid protease protease (acid)
Produces glucoamylase
Produces taka-amylase A
Cloning of the taka-amylase A gene
Cloning of the acetamidase gene
Cloning of the acid protease-encoding gene DDBJ D13894
Potential pigment producer

Preceptrol
No
Characteristics
Comments
Genome sequencing strain (National Institute of Technology and Evaluation (NITE), Japan).
Handling information
Medium
NTCC® Medium 200: YM agar or
NTCC® YM broth Medium 312: Czapek's agar
NTCC® Medium 336: Potato dextrose agar (PDA)
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

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